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The Synaptic Vesicle Priming Protein CAPS-1 Shapes the Adaptation of Sensory Evoked Responses in Mouse Visual Cortex
- Source :
- Cell Reports, Vol 30, Iss 10, Pp 3261-3269.e4 (2020), Cell Reports
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- Summary: Short-term plasticity gates information transfer across neuronal synapses and is thought to be involved in fundamental brain processes, such as cortical gain control and sensory adaptation. Neurons employ synaptic vesicle priming proteins of the CAPS and Munc13 families to shape short-term plasticity in vitro, but the relevance of this phenomenon for information processing in the intact brain is unknown. By combining sensory stimulation with in vivo patch-clamp recordings in anesthetized mice, we show that genetic deletion of CAPS-1 in thalamic neurons results in more rapid adaptation of sensory-evoked subthreshold responses in layer 4 neurons of the primary visual cortex. Optogenetic experiments in acute brain slices further reveal that the enhanced adaptation is caused by more pronounced short-term synaptic depression. Our data indicate that neurons engage CAPS-family priming proteins to shape short-term plasticity for optimal sensory information transfer between thalamic and cortical neurons in the intact brain in vivo. : Nestvogel et al. perform patch-clamp recordings of layer 4 neurons in the primary visual cortex of anesthetized mice to demonstrate that CAPS-family vesicle priming proteins control synaptic short-term plasticity and adaptation of sensory-evoked thalamocortical synaptic transmission, linking the action of dedicated vesicle priming proteins to sensory processing in vivo. Keywords: Synaptic Vesicle Priming, in-vivo patch clamp technique, Acute Brain slice Patch technique, Optogenetic Stimulation, Sensory Adaptation, Visual Cortex, Ca2+- dependent Activator Protein for Secretion (CAPS)
- Subjects :
- 0301 basic medicine
Sensation
Nerve Tissue Proteins
Sensory system
Plasticity
Optogenetics
Biology
Synaptic Transmission
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
0302 clinical medicine
medicine
Animals
Evoked Potentials
lcsh:QH301-705.5
Visual Cortex
Mice, Knockout
Neurons
Sensory Adaptation
Sensory stimulation therapy
Adaptation, Ocular
Calcium-Binding Proteins
030104 developmental biology
Visual cortex
medicine.anatomical_structure
lcsh:Biology (General)
Synaptic Vesicles
Neuroscience
Priming (psychology)
Gene Deletion
030217 neurology & neurosurgery
Synaptic vesicle priming
Subjects
Details
- ISSN :
- 22111247
- Volume :
- 30
- Database :
- OpenAIRE
- Journal :
- Cell Reports
- Accession number :
- edsair.doi.dedup.....f088470e621d7ba19ac98727fea5689b