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Potential of Melt Electrowritten Scaffolds Seeded with Meniscus Cells and Mesenchymal Stromal Cells

Authors :
Lucienne A. Vonk
Mylène de Ruijter
Jos Malda
Jasmijn V. Korpershoek
Miguel Castilho
Daniel B.F. Saris
Bastiaan F. Terhaard
Michella H. Hagmeijer
EAISI Health
ICMS Affiliated
Orthopaedic Biomechanics
Equine Musculoskeletal Biology
dES RMSC
Afd methoden en statistieken
Source :
International Journal of Molecular Sciences, Vol 22, Iss 11200, p 11200 (2021), International Journal of Molecular Sciences, International Journal of Molecular Sciences, 22(20), 1. MDPI AG, International Journal of Molecular Sciences, 22(20):11200. Multidisciplinary Digital Publishing Institute (MDPI), Volume 22, Issue 20
Publication Year :
2021
Publisher :
MDPI AG, 2021.

Abstract

Meniscus injury and meniscectomy are strongly related to osteoarthritis, thus there is a clinical need for meniscus replacement. The purpose of this study is to create a meniscus scaffold with micro-scale circumferential and radial fibres suitable for a one-stage cell-based treatment. Poly-caprolactone-based scaffolds with three different architectures were made using melt electrowriting (MEW) technology and their in vitro performance was compared with scaffolds made using fused-deposition modelling (FDM) and with the clinically used Collagen Meniscus Implants® (CMI®). The scaffolds were seeded with meniscus and mesenchymal stromal cells (MSCs) in fibrin gel and cultured for 28 d. A basal level of proteoglycan production was demonstrated in MEW scaffolds, the CMI®, and fibrin gel control, yet within the FDM scaffolds less proteoglycan production was observed. Compressive properties were assessed under uniaxial confined compression after 1 and 28 d of culture. The MEW scaffolds showed a higher Young’s modulus when compared to the CMI® scaffolds and a higher yield point compared to FDM scaffolds. This study demonstrates the feasibility of creating a wedge-shaped meniscus scaffold with MEW using medical-grade materials and seeding the scaffold with a clinically-feasible cell number and -type for potential translation as a one-stage treatment.

Details

Language :
English
ISSN :
16616596 and 14220067
Volume :
22
Issue :
11200
Database :
OpenAIRE
Journal :
International Journal of Molecular Sciences
Accession number :
edsair.doi.dedup.....f05337bf2e18d6c61ccf95b5eca7bb59