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Potential of Melt Electrowritten Scaffolds Seeded with Meniscus Cells and Mesenchymal Stromal Cells
- Source :
- International Journal of Molecular Sciences, Vol 22, Iss 11200, p 11200 (2021), International Journal of Molecular Sciences, International Journal of Molecular Sciences, 22(20), 1. MDPI AG, International Journal of Molecular Sciences, 22(20):11200. Multidisciplinary Digital Publishing Institute (MDPI), Volume 22, Issue 20
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- Meniscus injury and meniscectomy are strongly related to osteoarthritis, thus there is a clinical need for meniscus replacement. The purpose of this study is to create a meniscus scaffold with micro-scale circumferential and radial fibres suitable for a one-stage cell-based treatment. Poly-caprolactone-based scaffolds with three different architectures were made using melt electrowriting (MEW) technology and their in vitro performance was compared with scaffolds made using fused-deposition modelling (FDM) and with the clinically used Collagen Meniscus Implants® (CMI®). The scaffolds were seeded with meniscus and mesenchymal stromal cells (MSCs) in fibrin gel and cultured for 28 d. A basal level of proteoglycan production was demonstrated in MEW scaffolds, the CMI®, and fibrin gel control, yet within the FDM scaffolds less proteoglycan production was observed. Compressive properties were assessed under uniaxial confined compression after 1 and 28 d of culture. The MEW scaffolds showed a higher Young’s modulus when compared to the CMI® scaffolds and a higher yield point compared to FDM scaffolds. This study demonstrates the feasibility of creating a wedge-shaped meniscus scaffold with MEW using medical-grade materials and seeding the scaffold with a clinically-feasible cell number and -type for potential translation as a one-stage treatment.
- Subjects :
- Male
Scaffold
collagen meniscus implant®
meniscus injury
Meniscectomy18
Biofabrication
Tissue-engineering
02 engineering and technology
Meniscus (anatomy)
Extracellular Matrix/chemistry
Tissue engineering
meniscus
80 and over
Biology (General)
Melt electrowriting
Cells, Cultured
Spectroscopy
Aged, 80 and over
0303 health sciences
Cultured
Tissue Scaffolds
biology
Clinical translation
General Medicine
Middle Aged
021001 nanoscience & nanotechnology
musculoskeletal system
Extracellular Matrix
Computer Science Applications
Chemistry
meniscectomy18
medicine.anatomical_structure
Meniscus injury
Female
Proteoglycans
Seeding
0210 nano-technology
Adult
melt electrowriting
Materials science
QH301-705.5
Cells
Collagen meniscus implant®
Article
clinical translation
Catalysis
Fibrin
Inorganic Chemistry
Tissue Scaffolds/chemistry
03 medical and health sciences
Elastic Modulus
medicine
Humans
Meniscus
Physical and Theoretical Chemistry
Molecular Biology
QD1-999
Aged
030304 developmental biology
biofabrication
Organic Chemistry
Mesenchymal stem cell
Mesenchymal Stem Cells
Proteoglycans/metabolism
tissue-engineering
Proteoglycan
Meniscus/cytology
biology.protein
Biomedical engineering
Subjects
Details
- Language :
- English
- ISSN :
- 16616596 and 14220067
- Volume :
- 22
- Issue :
- 11200
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....f05337bf2e18d6c61ccf95b5eca7bb59