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Failure to detect the action of antidepressants in the forced swim test in Swiss mice
- Source :
- Acta Neuropsychiatrica. 30:158-167
- Publication Year :
- 2017
- Publisher :
- Cambridge University Press (CUP), 2017.
-
Abstract
- ObjectiveThe aims of this study were to replicate previously published experiments and to modify the protocol to detect the effects of chronic antidepressant treatment in mice.MethodsMale Swiss mice (n=6–8/group) housed in reversed light/dark cycle were randomly assigned into receive vehicle (10% sucrose), sub-effective doses (1 and 3 mg/kg) or effective doses (10 and 30 mg/kg) of bupropion, desipramine, and fluoxetine and a candidate antidepressant, sodium butyrate (1–30 mg/kg) per gavage (p.o.) 1 h before the forced swim test (FST). Treatments continued daily for 7 and 14 days during retests 1 and 2, respectively. In an additional experiment, mice received fluoxetine (20 mg/kg) or vehicle (10% sucrose or 0.9% saline) p.o. or i.p. before the FST. Mice housed in reversed or standard light/dark cycles received fluoxetine (20 mg/kg) prior FST. Video recordings of behavioural testing were used for blind assessment of the outcomes.ResultsAccording to the expected, doses of antidepressants considered sub-effective failed to affect the immobility time of mice in the FST. Surprisingly, acute and chronic treatment with the high doses of bupropion, desipramine, and fluoxetine or sodium butyrate also failed to reduce the immobility time of mice in the FST. Fluoxetine 20 mg/kg was also ineffective in the FST when injected i.p. or in mice housed in normal light/dark cycle.ConclusionData suggest the lack of efficacy of orally administered bupropion, desipramine, fluoxetine in the FST in Swiss mice. High variability, due to high and low immobility mice, may explain the limited effects of the treatments.
- Subjects :
- Male
medicine.medical_treatment
Motor Activity
Pharmacology
Mice
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Fluoxetine
Desipramine
medicine
Animals
Bupropion
Saline
Biological Psychiatry
Behavior, Animal
business.industry
Immobility Response, Tonic
Sodium butyrate
Antidepressive Agents
030227 psychiatry
Psychiatry and Mental health
chemistry
Butyric Acid
Antidepressant
Psychopharmacology
business
030217 neurology & neurosurgery
medicine.drug
Behavioural despair test
Subjects
Details
- ISSN :
- 16015215 and 09242708
- Volume :
- 30
- Database :
- OpenAIRE
- Journal :
- Acta Neuropsychiatrica
- Accession number :
- edsair.doi.dedup.....f04b7eef222fda1d747a3c69aec1a658