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At similar weight loss, dietary composition determines the degree of glycemic improvement in diet-induced obese C57BL/6 mice
- Source :
- PLoS ONE, PLoS ONE, Vol 13, Iss 7, p e0200779 (2018)
- Publication Year :
- 2018
-
Abstract
- BACKGROUND: Achieving weight loss is the cornerstone of the treatment of the metabolic consequences of obesity, in particular of glucose intolerance. OBJECTIVE: To determine whether improvement in glucose control depends on dietary macronutrient composition of the diet at identical weight loss. MATERIALS AND METHODS: Twenty-two weeks old diet-induced obese C57BL/6 mice lost weight through caloric restriction on normal chow (R-NC) or high fat diet (R-HF). Control mice were fed normal chow (LEAN) or high fat diet (OBESE) ad libitum. Body weight and composition were assessed after 8 weeks of dietary intervention. Glucose homeostasis was evaluated by intraperitoneal glucose tolerance tests (IPGTT). Epididymal white adipose (eWAT) and hepatic tissues were analyzed by immunohistochemistry and RT-qPCR. RESULTS: By 30 weeks of age, the body weight of the mice on R-NC (31.6±1.7g, mean±SEM) and R-HF (32.3±0.9g) was similar to LEAN mice (31.9±1.4g), while OBESE mice weighed 51.7±2.4g. Glucose tolerance in R-NC was better than in LEAN mice (69% AUC IPGTT, P 0.0168) whereas R-HF mice remained significantly less glucose tolerant (125% AUC IPGTT, P 0.0279 vs LEAN), despite identical weight loss. The eWAT pads and adipocyte size were similar in LEAN and R-NC mice, while the eWAT pad size of R-HF was 180% of R-NC (P < 0.0001) and the average adipocyte size of R-HF mice was 134% of R-NC fed mice (P 0.0285). No LEAN or R-NC mice had hepatic steatosis, in contrast to 28.6% of R-HF mice. Compared to OBESE mice, inflammatory markers were lower in eWAT and liver tissue of R-NC, but not in R-HF mice. Measures of visceral adiposity correlated well with glucose tolerance parameters. CONCLUSIONS: In mice, caloric restriction on a normal chow diet improved glucose tolerance significantly more when identical weight loss was achieved on a high fat diet. ispartof: PLOS ONE vol:13 issue:7 ispartof: location:United States status: published
- Subjects :
- 0301 basic medicine
Blood Glucose
Male
Steatosis
Physiology
Adipose tissue
lcsh:Medicine
Mice, Obese
Pathology and Laboratory Medicine
Biochemistry
Fats
Cytopathology
chemistry.chemical_compound
Eating
Mice
0302 clinical medicine
Endocrinology
Weight loss
Animal Cells
Adipocyte
Adipocytes
Medicine and Health Sciences
Medicine
Glucose homeostasis
Homeostasis
lcsh:Science
Adiposity
Connective Tissue Cells
2. Zero hunger
INSULIN-RESISTANCE
Multidisciplinary
Liver Diseases
Fatty liver
Lipids
Type 2 Diabetes
Multidisciplinary Sciences
ADIPOSE-TISSUE
Adipose Tissue
Physiological Parameters
Connective Tissue
Body Composition
Science & Technology - Other Topics
medicine.symptom
Cellular Types
Anatomy
Research Article
medicine.medical_specialty
Endocrine Disorders
Adipose Tissue, White
POUNDS LOST
030209 endocrinology & metabolism
Gastroenterology and Hepatology
Calorimetry
Diet, High-Fat
03 medical and health sciences
Internal medicine
Glucose Intolerance
Weight Loss
Diabetes Mellitus
Animals
Obesity
Caloric Restriction
Nutrition
Inflammation
Science & Technology
business.industry
lcsh:R
Body Weight
Biology and Life Sciences
Nutrients
Cell Biology
medicine.disease
Dietary Fats
Diet
Fatty Liver
Mice, Inbred C57BL
030104 developmental biology
Glucose
Biological Tissue
chemistry
Diet and Type 2 Diabetes
CLINICAL-PRACTICE
FAT
Anatomical Pathology
Metabolic Disorders
lcsh:Q
business
Diet-induced obese
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 13
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.doi.dedup.....f03a8f4d9ab14843039fe25f092ddbb5