Adoptive Transfer of Basophils Enriched from Mouse Spleen

CD49b is a member of the integrin family, expressed on basophils, natural killer (NK) cells and a subset CD4(+) T cells in the spleen. This protocol describes the adoptive transfer of basophil-enriched CD49b(+) cells obtained from mouse spleens by magnetic enrichment. This protocol can be used to assess the contribution of basophils or basophil-derived mediators to a certain immune response.