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Development of polyvinylpyrrolidone/paclitaxel self-assemblies for breast cancer
- Source :
- Acta Pharmaceutica Sinica B, Vol 8, Iss 4, Pp 602-614 (2018), Acta Pharmaceutica Sinica. B
- Publication Year :
- 2018
- Publisher :
- Elsevier, 2018.
-
Abstract
- The goal of this investigation was to develop and demonstrate a polymer/paclitaxel self-assembly (PTX-SA) formulation. Polymer/PTX-SAs were screened based on smaller size of formulation using dynamic light scattering analysis. Additionally, fluorescence microscopy and flow cytometry studies exhibited that polyvinylpyrrolidone (PVP)-based PTX-SAs (PVP/PTX-SAs) had superior cellular internalization capability in MCF7 and MDA-MB-231 breast cancer cells. The optimized PVP/PTX-SAs exhibited less toxicity to human red blood cells indicating a suitable formulation for reducing systemic toxicity. The formation of PVP and PTX self-assemblies was confirmed using fluorescence quenching and transmission electron microscopy which indicated that the PVP/PTX-SAs were spherical in shape with an average size range of 53.81 nm as detected by transmission electron microscopy (TEM). FTIR spectral analysis demonstrates incorporation of polymer and paclitaxel functional groups in PVP/PTX-SAs. Both proliferation (MTS) and clonogenic (colony formation) assays were used to validate superior anticancer activity of PVP/PTX-SAs in breast cancer cells over paclitaxel. Such superior anticancer activity was also demonstrated by downregulation of the expression of pro-survival protein (Bcl-xL), upregulation of apoptosis-associated proteins (Bid, Bax, cleaved caspase 7, and cleaved PARP) and β-tubulin stabilization. These results support the hypothesis that PVP/PTX-SAs improved paclitaxel delivery to cancer cells.<br />Graphical abstract An optimized polyvinylpyrrolidone/paclitaxel self-assembly formulation was established and tested for its improved cellular binding and anti-cancer activity against breast cancer cells.fx1
- Subjects :
- 02 engineering and technology
macromolecular substances
Flow cytometry
03 medical and health sciences
chemistry.chemical_compound
Breast cancer
0302 clinical medicine
Dynamic light scattering
medicine
Fluorescence microscope
General Pharmacology, Toxicology and Pharmaceutics
Polymer
Clonogenic assay
Self-assemblies
Polyvinylpyrrolidone
medicine.diagnostic_test
Chemistry
lcsh:RM1-950
technology, industry, and agriculture
021001 nanoscience & nanotechnology
3. Good health
lcsh:Therapeutics. Pharmacology
Paclitaxel
030220 oncology & carcinogenesis
Drug delivery
Cancer cell
Biophysics
Nanoparticles
Original Article
Cancer therapeutics
0210 nano-technology
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 22113835
- Volume :
- 8
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Acta Pharmaceutica Sinica B
- Accession number :
- edsair.doi.dedup.....f031bf5764a30f5c1af4bda0a558f685