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Co-Expression Networks Reveal Potential Regulatory Roles of miRNAs in Fatty Acid Composition of Nelore Cattle
- Source :
- Frontiers in Genetics, Vol 10 (2019), Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice), Empresa Brasileira de Pesquisa Agropecuária (Embrapa), instacron:EMBRAPA, Frontiers in Genetics
- Publication Year :
- 2019
- Publisher :
- Frontiers Media S.A., 2019.
-
Abstract
- Fatty acid (FA) content affects the sensorial and nutritional value of meat and plays a significant role in biological processes such as adipogenesis and immune response. It is well known that, in beef, the main FAs associated with these biological processes are oleic acid (C18:1 cis9, OA) and conjugated linoleic acid (CLA-c9t11), which may have beneficial effects on metabolic diseases such as type 2 diabetes and obesity. Here, we performed differential expression and co-expression analyses, weighted gene co-expression network analysis (WGCNA) and partial correlation with information theory (PCIT), to uncover the complex interactions between miRNAs and mRNAs expressed in skeletal muscle associated with FA content. miRNA and mRNA expression data were obtained from skeletal muscle of Nelore cattle that had extreme genomic breeding values for OA and CLA. Insulin and MAPK signaling pathways were identified by WGCNA as central pathways associated with both of these fatty acids. Co-expression network analysis identified bta-miR-33a/b, bta-miR-100, bta-miR-204, bta-miR-365-5p, btamiR-660, bta-miR-411a, bta-miR-136, bta-miR-30-5p, bta-miR-146b, bta-let-7a-5p, bta-let-7f, bta-let-7, bta-miR 339, bta-miR-10b, bta-miR 486, and the genes ACTA1 and ALDOA as potential regulators of fatty acid synthesis. This study provides evidence and insights into the molecular mechanisms and potential target genes involved in fatty acid content differences in Nelore beef cattle, revealing new candidate pathways of phenotype modulation that could positively benefit beef production and human consumption. Made available in DSpace on 2019-07-25T01:02:05Z (GMT). No. of bitstreams: 1 APCoexpressionnetworks.pdf: 1517209 bytes, checksum: c6c99bbfd9721e2c08e0d765e8b03b88 (MD5) Previous issue date: 2019 Article 651. Na publicação: Adhemar Zerlotini, Luciana C.A. Regitano.
- Subjects :
- 0301 basic medicine
lcsh:QH426-470
Conjugated linoleic acid
Redes de co-expressão
mRNA
Ácido linoleico conjugado
Bos indicus
Beef cattle
Biology
conjugated linoleic acid
03 medical and health sciences
chemistry.chemical_compound
integrative genomics
0302 clinical medicine
Ácidos graxos
Genetics
Genômica
MRNA
Gene
Genetics (clinical)
Fatty acid synthesis
Original Research
miRNA
chemistry.chemical_classification
Gado de Corte
Bos Indicus
Ácido oleico
Fatty acid
Phenotype
Oleic acid
lcsh:Genetics
030104 developmental biology
chemistry
Biochemistry
oleic acid
Adipogenesis
030220 oncology & carcinogenesis
Integrative genomics
Molecular Medicine
MiRNA
Subjects
Details
- Language :
- English
- ISSN :
- 16648021
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Frontiers in Genetics
- Accession number :
- edsair.doi.dedup.....f0316f9460e31f25adae916b5363f788