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Co-Expression Networks Reveal Potential Regulatory Roles of miRNAs in Fatty Acid Composition of Nelore Cattle

Authors :
Aline Silva Mello Cesar
Bruno G. N. Andrade
Luciana Correia de Almeida Regitano
Priscila S. N. de Oliveira
Wellison Jarles da Silva Diniz
James E. Koltes
Adhemar Zerlotini
Marcela Maria de Souza
Gerson Barreto Mourão
James M. Reecy
Luiz Lehmann Coutinho
PRISCILA S. N. DE OLIVEIRA, CPPSE
LUIZ L. COUTINHO, Esalq/USP
ALINE S. M. CESAR, Esalq/USP
WELLISON J. DA SILVA DINIZ, UFSCar
MARCELA M. DE SOUZA, Iowa State University
BRUNO G. ANDRADE, CPPSE
JAMES E. KOLTES, Iowa State University
GERSON B. MOURÃO, Esalq/USP
ADHEMAR ZERLOTINI NETO, CNPTIA
JAMES M. REECY, Iowa State University
LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE.
Source :
Frontiers in Genetics, Vol 10 (2019), Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice), Empresa Brasileira de Pesquisa Agropecuária (Embrapa), instacron:EMBRAPA, Frontiers in Genetics
Publication Year :
2019
Publisher :
Frontiers Media S.A., 2019.

Abstract

Fatty acid (FA) content affects the sensorial and nutritional value of meat and plays a significant role in biological processes such as adipogenesis and immune response. It is well known that, in beef, the main FAs associated with these biological processes are oleic acid (C18:1 cis9, OA) and conjugated linoleic acid (CLA-c9t11), which may have beneficial effects on metabolic diseases such as type 2 diabetes and obesity. Here, we performed differential expression and co-expression analyses, weighted gene co-expression network analysis (WGCNA) and partial correlation with information theory (PCIT), to uncover the complex interactions between miRNAs and mRNAs expressed in skeletal muscle associated with FA content. miRNA and mRNA expression data were obtained from skeletal muscle of Nelore cattle that had extreme genomic breeding values for OA and CLA. Insulin and MAPK signaling pathways were identified by WGCNA as central pathways associated with both of these fatty acids. Co-expression network analysis identified bta-miR-33a/b, bta-miR-100, bta-miR-204, bta-miR-365-5p, btamiR-660, bta-miR-411a, bta-miR-136, bta-miR-30-5p, bta-miR-146b, bta-let-7a-5p, bta-let-7f, bta-let-7, bta-miR 339, bta-miR-10b, bta-miR 486, and the genes ACTA1 and ALDOA as potential regulators of fatty acid synthesis. This study provides evidence and insights into the molecular mechanisms and potential target genes involved in fatty acid content differences in Nelore beef cattle, revealing new candidate pathways of phenotype modulation that could positively benefit beef production and human consumption. Made available in DSpace on 2019-07-25T01:02:05Z (GMT). No. of bitstreams: 1 APCoexpressionnetworks.pdf: 1517209 bytes, checksum: c6c99bbfd9721e2c08e0d765e8b03b88 (MD5) Previous issue date: 2019 Article 651. Na publicação: Adhemar Zerlotini, Luciana C.A. Regitano.

Details

Language :
English
ISSN :
16648021
Volume :
10
Database :
OpenAIRE
Journal :
Frontiers in Genetics
Accession number :
edsair.doi.dedup.....f0316f9460e31f25adae916b5363f788