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Ultrasensitive behavior in the synthesis of storage polysaccharides in cyanobacteria
- Source :
- Planta. 216:969-975
- Publication Year :
- 2003
- Publisher :
- Springer Science and Business Media LLC, 2003.
-
Abstract
- The glycogen synthetic pathway operates ultrasensitively as a function of the ADPglucose pyrophosphorylase (ADPGlcPPase) allosteric effectors, 3-phosphoglycerate and Pi, in permeabilized cells of the cyanobacterium Anabaena PCC 7120. In vitro data previously showed that the ultrasensitive behavior of ADPGlcPPase depends upon cross-talk between the two allosteric effectors, the enzyme's response being additionally modulated by molecular crowding [D.F. Gómez Casatiet al. (2000) Biochem J 350:139–147]. In the present work we show, experimentally and with a mathematical model, that α-1,4-glucan synthesis is also ultrasensitive in cells due to the propagation of the switch-like behavior of ADPGlcPPase to the synthetic pathway. Amplifications of up to 20-fold in storage-polysaccharide synthesis can be achieved with a modest 6.7-fold increase in 3-phosphoglycerate in the presence of 5 mM Pi in contrast to the 30-fold necessary in its absence. This is the first time that this phenomenon has been reported to occur in the glycogen synthetic pathway of a photosynthetic prokaryote. The implications of the results for plant cell physiology during light–dark transitions are discussed. Fil: Gomez Casati, Diego Fabian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Cortassa, Sonia del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina Fil: Aon, Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina Fil: Iglesias, Alberto Alvaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
- Subjects :
- ANABAENA
Cell Membrane Permeability
Allosteric regulation
Glucose-1-Phosphate Adenylyltransferase
Plant Science
Biology
Glyceric Acids
Models, Biological
Ciencias Biológicas
Adenosine Diphosphate Glucose
chemistry.chemical_compound
Genetics
Glucans
MOLECULAR CROWDING
chemistry.chemical_classification
Glycogen
Effector
Anabaena
Glucosephosphates
Permeabilized cyanobacteria
ADPGLUCOSE PYROPHOSPHORYLASE
Metabolism
Bioquímica y Biología Molecular
Plant cell
biology.organism_classification
Nucleotidyltransferases
Enzyme
Biochemistry
chemistry
CIENCIAS NATURALES Y EXACTAS
Subjects
Details
- ISSN :
- 14322048 and 00320935
- Volume :
- 216
- Database :
- OpenAIRE
- Journal :
- Planta
- Accession number :
- edsair.doi.dedup.....f002be087594eb8e78f8ae74f0dc0479