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DNA Methylation Is Crucial for the Early Development in the Oyster C. gigas
- Source :
- Marine Biotechnology, Marine Biotechnology, Springer Verlag, 2013, 15 (6), pp.739-753. ⟨10.1007/s10126-013-9523-2⟩
- Publication Year :
- 2013
- Publisher :
- HAL CCSD, 2013.
-
Abstract
- International audience; In vertebrates, epigenetic modifications influence gene transcription, and an appropriate DNA methylation is critical in development. Indeed, a precise temporal and spatial pattern of early gene expression is mandatory for a normal embryogenesis. However, such a regulation and its underlying mechanisms remain poorly understood in more distant organisms such as Lophotrochozoa. Thus, despite DNA in the oyster genome being methylated, the role of DNA methylation in development is unknown. To clarify this point, oyster genomic DNA was examined during early embryogenesis and found differentially methylated. Reverse transcriptase quantitative polymerase chain reaction indicated stage-specific levels of transcripts encoding DNA-methyltransferase (DNMT) and methyl-binding domain proteins. In addition, as highlighted by electronic microscopy and immunohistochemistry, the DNMT inhibitor 5-aza-cytidine induced alterations in the quantity and the localisation of methylated DNA and severe dose-dependent development alterations and was lethal after zygotic genome reinitiation. Furthermore, methyl-DNA-immunoprecipitation–quantitative polymerase chain reaction revealed that the transcription level of most of the homeobox gene orthologues examined, but not of the other early genes investigated, was inversely correlated with their specific DNA methylation. Altogether, our results demonstrate that DNA methylation influences gene expression in Crassostrea gigas and is critical for oyster development, possibly by specifically controlling the transcription level of homeobox orthologues. These findings provide evidence for the importance of epigenetic regulation of development in Lophotrochozoans and bring new insights into the early life of C. gigas, one of the most important aquaculture resources worldwide.
- Subjects :
- Evolution
[SDV]Life Sciences [q-bio]
Enzyme-Linked Immunosorbent Assay
Aquaculture
Biology
Development
Applied Microbiology and Biotechnology
Methylation
Epigenesis, Genetic
03 medical and health sciences
0302 clinical medicine
Epigenetics of physical exercise
Transcription (biology)
Animals
Immunoprecipitation
Homeobox
14. Life underwater
Epigenetics
DNA Modification Methylases
Gene
RNA-Directed DNA Methylation
030304 developmental biology
Epigenomics
Homeodomain Proteins
Genetics
Analysis of Variance
0303 health sciences
Dose-Response Relationship, Drug
Reverse Transcriptase Polymerase Chain Reaction
Gene Expression Regulation, Developmental
DNA Methylation
Immunohistochemistry
Ostreidae
Real-time polymerase chain reaction
DNA methylation
Azacitidine
Microscopy, Electron, Scanning
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 14362228 and 14362236
- Database :
- OpenAIRE
- Journal :
- Marine Biotechnology, Marine Biotechnology, Springer Verlag, 2013, 15 (6), pp.739-753. ⟨10.1007/s10126-013-9523-2⟩
- Accession number :
- edsair.doi.dedup.....eff50d1c073c532ccb15e8bdfd89f3a9
- Full Text :
- https://doi.org/10.1007/s10126-013-9523-2⟩