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Functional lipidomics in patients on home parenteral nutrition: Effect of lipid emulsions
- Source :
- World Journal of Gastroenterology
- Publication Year :
- 2016
-
Abstract
- AIM To investigate the fatty acid-based functional lipidomics of patients on long-term home parenteral nutrition receiving different intravenous lipid emulsions. METHODS A cross-sectional comparative study was carried out on 3 groups of adults on home parenteral nutrition (HPN), receiving an HPN admixture containing an olive-soybean oil-based intravenous lipid emulsion (IVLE) (OO-IVLE; n = 15), a soybean- medium-chain triacylglycerol-olivefish oil-based IVLE (SMOF-IVLE; n = 8) or HPN without IVLE (No-IVLE; n = 8) and 42 healthy controls (HCs). The inclusion criteria were: duration of HPN ⥠3 mo, current HPN admixtures ⥠2 mo and HPN infusions ⥠2/wk. Blood samples were drawn 4-6 h after the discontinuation of the overnight HPN infusion. The functional lipidomics panel included: the red blood cell (RBC) fatty acid (FA) profile, molecular biomarkers [membrane fluidity: saturated/monounsaturated FA ratio = saturated fatty acid (SFA)/monounsaturated fatty acid (MUFA) index; inflammatory risk: n-6/n-3 polyunsaturated fatty acid (PUFA) ratio = n-6/n-3 index; cardiovascular risk: sum of n-3 eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) = n-3 index; free radical stress: sum of FA trans isomers = %trans index] and FA pathway enzyme activity estimate (delta-9-desaturase = D9D; delta-6-desaturase = D6D; delta-5-desaturase = D5D; elongase = ELO). Statistics were carried out using nonparametric tests. The amount of each FA was calculated as a percentage of the total FA content (relative%). RESULTS In the OO-IVLE group, the percentage of oleic acid in the RBCs was positively correlated with the weekly load of OO-IVLE (r = 0.540, p = 0.043). In the SMOFIVLE cohort, the RBC membrane EPA and DHA were positively correlated with the daily amount of SMOFIVLE (r = 0.751, p = 0.044) and the number of HPN infusions per week (r = 0.753; p = 0.046), respectively. The SMOF-IVLE group showed the highest EPA and DHA and the lowest arachidonic acid percentages (p < 0.001). The RBC membrane linoleic acid content was lower, and oleic and vaccenic acids were higher in all the HPN groups in comparison to the HCs. Vaccenic acid was positively correlated with the weekly HPN load of glucose in both the OO-IVLE (r = 0.716; p = 0.007) and the SMOF-IVLE (r = 0.732; p = 0.053) groups. The estimated activity of D9D was higher in all the HPN groups than in the HCs (p < 0.001). The estimated activity of D5D was lower in the SMOF-IVLE group than in the HCs (p = 0.013). The SFA/MUFA ratio was lower in all the HPN groups than in the HCs (p < 0.001). The n-6/n-3 index was lower and the n-3 index was higher in the SMOF-IVLE group in comparison to the HCs and to the other HPN groups (p < 0.001). The %trans index did not differ among the four groups. CONCLUSION The FA profile of IVLEs significantly influenced the cell membrane functional lipidomics. The amount of glucose in the HPN may play a relevant role, mediated by the insulin regulation of the FA pathway enzyme activities.
- Subjects :
- 0301 basic medicine
Male
Erythrocytes
Vaccenic acid
chemistry.chemical_compound
0302 clinical medicine
Risk Factors
Intravenous lipid emulsion
chemistry.chemical_classification
Chemistry
Fatty Acids
Gastroenterology
General Medicine
Chronic intestinal failure
Middle Aged
Eicosapentaenoic acid
Biochemistry
Docosahexaenoic acid
Cardiovascular Diseases
Saturated fatty acid
Metabolome
030211 gastroenterology & hepatology
Female
Parenteral Nutrition, Home
Polyunsaturated fatty acid
Adult
medicine.medical_specialty
Fat Emulsions, Intravenous
Adolescent
Docosahexaenoic Acids
Linoleic acid
Observational Study
Functional lipidomic
Cell membrane lipidome
03 medical and health sciences
Young Adult
Cell membrane fatty acid profile
Internal medicine
medicine
Humans
Metabolomics
Aged
Home parenteral nutrition
030109 nutrition & dietetics
Fatty acid
Lipid Metabolism
Functional lipidomics
Oleic acid
Intestinal Diseases
Endocrinology
Cross-Sectional Studies
Glucose
Chronic Disease
Biomarkers
Subjects
Details
- ISSN :
- 22192840
- Volume :
- 23
- Issue :
- 25
- Database :
- OpenAIRE
- Journal :
- World journal of gastroenterology
- Accession number :
- edsair.doi.dedup.....efe96a97208f3921b0bd2fdb27fffa39