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Spermatogonial deubiquitinase USP9X is essential for proper spermatogenesis in mice

Authors :
Hitomi Suzuki
Kasane Kishi
Yoshiakira Kanai
Masamichi Kurohmaru
Naoki Tsunekawa
Masami Kanai-Azuma
Aya Uchida
Hinako M. Takase
Stephen A. Wood
Source :
Reproduction. 154:135-143
Publication Year :
2017
Publisher :
Bioscientifica, 2017.

Abstract

USP9X (ubiquitin-specific peptidase 9, X chromosome) is the mammalian orthologue ofDrosophiladeubiquitinase fat facets that was previously shown to regulate the maintenance of the germ cell lineage partially through stabilizing Vasa, one of the widely conserved factors crucial for gametogenesis. Here, we demonstrate that USP9X is expressed in the gonocytes and spermatogonia in mouse testes from newborn to adult stages. By usingVasa-Cremice, germ cell-specific conditional deletion ofUsp9xfrom the embryonic stage showed no abnormality in the developing testes by 1 week and no appreciable defects in the undifferentiated and differentiating spermatogonia at postnatal and adult stages. Interestingly, after 2 weeks,Usp9x-null spermatogenic cells underwent apoptotic cell death at the early spermatocyte stage, and then, caused subsequent aberrant spermiogenesis, which resulted in a complete infertility ofUsp9xconditional knockout male mice. These data provide the first evidence of the crucial role of the spermatogonial USP9X during transition from the mitotic to meiotic phases and/or maintenance of early meiotic phase inUsp9xconditional knockout testes.

Details

ISSN :
17417899 and 14701626
Volume :
154
Database :
OpenAIRE
Journal :
Reproduction
Accession number :
edsair.doi.dedup.....efe3b27cf8173e48a0cdf6ce0579f30e
Full Text :
https://doi.org/10.1530/rep-17-0184