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Spermatogonial deubiquitinase USP9X is essential for proper spermatogenesis in mice
- Source :
- Reproduction. 154:135-143
- Publication Year :
- 2017
- Publisher :
- Bioscientifica, 2017.
-
Abstract
- USP9X (ubiquitin-specific peptidase 9, X chromosome) is the mammalian orthologue ofDrosophiladeubiquitinase fat facets that was previously shown to regulate the maintenance of the germ cell lineage partially through stabilizing Vasa, one of the widely conserved factors crucial for gametogenesis. Here, we demonstrate that USP9X is expressed in the gonocytes and spermatogonia in mouse testes from newborn to adult stages. By usingVasa-Cremice, germ cell-specific conditional deletion ofUsp9xfrom the embryonic stage showed no abnormality in the developing testes by 1 week and no appreciable defects in the undifferentiated and differentiating spermatogonia at postnatal and adult stages. Interestingly, after 2 weeks,Usp9x-null spermatogenic cells underwent apoptotic cell death at the early spermatocyte stage, and then, caused subsequent aberrant spermiogenesis, which resulted in a complete infertility ofUsp9xconditional knockout male mice. These data provide the first evidence of the crucial role of the spermatogonial USP9X during transition from the mitotic to meiotic phases and/or maintenance of early meiotic phase inUsp9xconditional knockout testes.
- Subjects :
- Male
0301 basic medicine
Embryology
Genotype
Spermiogenesis
Apoptosis
Spermatocyte
Biology
03 medical and health sciences
Endocrinology
Gonocyte
Endopeptidases
Testis
Conditional gene knockout
medicine
Animals
Spermatogenesis
Mitosis
Infertility, Male
Gametogenesis
Mice, Knockout
Age Factors
Obstetrics and Gynecology
Cell Biology
Spermatogonia
Cell biology
Mice, Inbred C57BL
Meiosis
Fertility
Phenotype
030104 developmental biology
medicine.anatomical_structure
Reproductive Medicine
Ubiquitin Thiolesterase
Germ cell
Signal Transduction
Subjects
Details
- ISSN :
- 17417899 and 14701626
- Volume :
- 154
- Database :
- OpenAIRE
- Journal :
- Reproduction
- Accession number :
- edsair.doi.dedup.....efe3b27cf8173e48a0cdf6ce0579f30e
- Full Text :
- https://doi.org/10.1530/rep-17-0184