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Nanopore-based technologies beyond DNA sequencing

Authors :
Yi-Lun Ying
Zheng-Li Hu
Shengli Zhang
Yujia Qing
Alessio Fragasso
Giovanni Maglia
Amit Meller
Hagan Bayley
Cees Dekker
Yi-Tao Long
Source :
Nature Nanotechnology. 17:1136-1146
Publication Year :
2022
Publisher :
Springer Science and Business Media LLC, 2022.

Abstract

Inspired by the biological processes of molecular recognition and transportation across membranes, nanopore techniques have evolved in recent decades as ultrasensitive analytical tools for individual molecules. In particular, nanopore-based single-molecule DNA/RNA sequencing has advanced genomic and transcriptomic research due to the portability, lower costs and long reads of these methods. Nanopore applications, however, extend far beyond nucleic acid sequencing. In this Review, we present an overview of the broad applications of nanopores in molecular sensing and sequencing, chemical catalysis and biophysical characterization. We highlight the prospects of applying nanopores for single-protein analysis and sequencing, single-molecule covalent chemistry, clinical sensing applications for single-molecule liquid biopsy, and the use of synthetic biomimetic nanopores as experimental models for natural systems. We suggest that nanopore technologies will continue to be explored to address a number of scientific challenges as control over pore design improves.

Details

ISSN :
17483395 and 17483387
Volume :
17
Database :
OpenAIRE
Journal :
Nature Nanotechnology
Accession number :
edsair.doi.dedup.....efd40bcbd91f24aec5745fd826b74f6f