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Adventitial growth and lung connective tissue growth factor expression in pulmonary arterioles due to hypobaric hypoxia in broilers
- Source :
- Poultry Science, Vol 99, Iss 4, Pp 1832-1837 (2020), Poultry Science
- Publication Year :
- 2020
- Publisher :
- Elsevier, 2020.
-
Abstract
- Forty broilers maintained under natural hypobaric hypoxia (2,638 m above sea level) and 20 maintained under relative normoxia (460 m above sea level) were selected as pulmonary hypertensive (PHB) and nonpulmonary hypertensive (NPHB), to estimate the degree of the adventitial vascular thickness in lung arterioles and connective tissue growth factor (CTGF) expression in lung. In each group, the adventitial thickness (%AT) of 20 arterioles with 100 to 250 μm of external diameter was measured in lung samples of 24 and 42-day-old broilers. Also, mRNA extraction and real-time reverse transcription-PCR analysis were used to measure lung CTGF expression. The %AT was higher in PHB at 42 D as compared to NPHB at both ages and PHB at 24 D; however, the same differences were not evidenced at 24 D. In the 2 ages evaluated, differences were observed in the %AT between broilers under hypobaric hypoxia (PHB and NPHB) and under relative normoxia (P < 0.01). In broilers subjected to relative normoxia, no significant differences were found at any of the 2 ages. The expression levels of CTGF mRNA were higher in PHB compared to NPHB at the 2 ages. The %AT was higher in PHB with high levels of expression of CTGF mRNA than those NPHB with low expression of CTGF mRNA. This study showed that adventitial thickening is part of the pulmonary hypertension (PH) physiopathology in broilers exposed to hypobaric hypoxia, in which CTGF appears to be a fibrosis enhancer. Although present data suggest that adventitial engrossment could be a time-dependent process, individual susceptibility and the variable time-course of PH pathophysiology have to be considered.
- Subjects :
- Male
medicine.medical_treatment
Gene Expression
TGF-β, transforming growth factor-beta
PHB, pulmonary hypertensive broilers
NPHB, nonpulmonary hypertensive broilers
Fibrosis
pulmonary hypertension
Hypoxia
Lung
lcsh:SF1-1100
0303 health sciences
Chemistry
04 agricultural and veterinary sciences
General Medicine
masl, meters above sea level
Pathophysiology
Arterioles
medicine.anatomical_structure
medicine.medical_specialty
Hypertension, Pulmonary
vascular remodeling
PH, pulmonary hypertension
Connective tissue
HPRT, hypoxanthine guanine phosphoribosyltransferase
broiler
CTGF, connective tissue growth factor
Avian Proteins
03 medical and health sciences
AT, adventitial thickness
Internal medicine
medicine
ET-1, endothelin-1
Animals
CI, cardiac index
Poultry Diseases
030304 developmental biology
NO, nitric oxide
Molecular and Cellular Biology
Growth factor
0402 animal and dairy science
Broiler
Connective Tissue Growth Factor
medicine.disease
hypobaric hypoxia
040201 dairy & animal science
Pulmonary hypertension
CTGF
Endocrinology
Animal Science and Zoology
lcsh:Animal culture
Chickens
Subjects
Details
- Language :
- English
- ISSN :
- 00325791
- Volume :
- 99
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Poultry Science
- Accession number :
- edsair.doi.dedup.....efd0e4b241695ba79dc56ae54dc1eda1