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Analysis of mRNA, miRNA, and DNA in Bone Cells by RT-qPCR and In Situ Hybridization
- Source :
- Methods in molecular biology, Methods in molecular biology, 1914, pp.169-196, 2019, ⟨10.1007/978-1-4939-8997-3_9⟩, Methods in Molecular Biology ISBN: 9781493989966
- Publication Year :
- 2019
- Publisher :
- HAL CCSD, 2019.
-
Abstract
- International audience; The aim of this chapter is to describe a method used to evaluate gene expression and microRNAs (miRNAs) in bone cells or tissue using Reverse transcription and quantitative Polymerase Chain Reaction (RT-qPCR), and a method to assess chromogenic in situ hybridization (CISH) on Formalin Fixed Paraffin Embedded (FFPE ) mouse bone tissue to detect both DNA and mRNA transcripts using the double digoxigenin (DIG) locked nucleic acid (LNA™) probes .
- Subjects :
- 0301 basic medicine
MESH: Xenograft Model Antitumor Assays
Histology
MESH: Cell Line, Tumor
mRNA
[SDV]Life Sciences [q-bio]
Chromogenic in situ hybridization
In situ hybridization
03 medical and health sciences
chemistry.chemical_compound
MESH: Gene Expression Profiling
0302 clinical medicine
MESH: In Situ Hybridization
MESH: Oligonucleotides
Bone sarcoma
MESH: Reverse Transcriptase Polymerase Chain Reaction
Bone cell
MESH: Mice, Nude
Digoxigenin
MESH: Animals
Locked nucleic acid
CISH
Bone
MESH: Mice
miRNA
MESH: RNA, Messenger
LNA probes
MESH: Humans
Chemistry
MESH: Real-Time Polymerase Chain Reaction
RT-qPCR
MESH: Digoxigenin
MESH: DNA
DNA
MESH: Bone and Bones
Molecular biology
Reverse transcriptase
3. Good health
Primers
MESH: Histocytological Preparation Techniques
030104 developmental biology
Real-time polymerase chain reaction
030220 oncology & carcinogenesis
Gene expression
MESH: MicroRNAs
Subjects
Details
- Language :
- English
- ISBN :
- 978-1-4939-8996-6
- ISBNs :
- 9781493989966
- Database :
- OpenAIRE
- Journal :
- Methods in molecular biology, Methods in molecular biology, 1914, pp.169-196, 2019, ⟨10.1007/978-1-4939-8997-3_9⟩, Methods in Molecular Biology ISBN: 9781493989966
- Accession number :
- edsair.doi.dedup.....efbb2f42bff8de6d0385b4c93cb1e9ed