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Enigma Plays Roles in Survival of Thyroid Carcinoma Cells through PI3K/AKT Signaling and Survivin
- Source :
- Anticancer Research. 38:3515-3525
- Publication Year :
- 2018
- Publisher :
- Anticancer Research USA Inc., 2018.
-
Abstract
- Background/aim The aim of the present study was to assess the role of enigma protein in survival of thyroid carcinoma cells. Materials and methods BCPAP and 8505C human thyroid carcinoma cells were used. Cell viability using CCK-8 assay, the percentage of dead cells using trypan blue assay, cytotoxic activity using cytotoxicity assay, cell growth rate and cell migration using wound-healing assay were performed. Results In enigma siRNA-transfected cells, cell viability, and the protein levels of AKT and survivin decreased. The percentage of dead cells, cytotoxic activity and cleaved poly (ADP-ribose) polymerase (PARP) protein levels increased. After transfection of p110α plasmid, the alterations in cell viability, the percentage of dead cells, cytotoxic activity, and protein levels of AKT, survivin and cleaved PARP were abrogated. Cell growth rate and cell migration were reduced with reduction of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) protein levels, as well as increased p53 and p21 protein levels. Conclusion Enigma affects cell survival through modulation of phosphatidylinositol-3 kinase/AKT signaling and survivin, and regulates cell proliferation and migration via involvement of MMP-2, MMP-9, p53 and p21 in thyroid carcinoma cells.
- Subjects :
- Cyclin-Dependent Kinase Inhibitor p21
0301 basic medicine
Cancer Research
Cell Survival
Survivin
Inhibitor of Apoptosis Proteins
Thyroid carcinoma
Phosphatidylinositol 3-Kinases
03 medical and health sciences
Cell Movement
Cell Line, Tumor
Humans
Cytotoxic T cell
Thyroid Neoplasms
Viability assay
Protein kinase B
Adaptor Proteins, Signal Transducing
Cell Proliferation
Cell growth
Chemistry
Cell migration
General Medicine
Transfection
LIM Domain Proteins
Cytoskeletal Proteins
030104 developmental biology
Matrix Metalloproteinase 9
Oncology
Cancer research
Matrix Metalloproteinase 2
RNA Interference
Poly(ADP-ribose) Polymerases
Tumor Suppressor Protein p53
Proto-Oncogene Proteins c-akt
Signal Transduction
Subjects
Details
- ISSN :
- 17917530 and 02507005
- Volume :
- 38
- Database :
- OpenAIRE
- Journal :
- Anticancer Research
- Accession number :
- edsair.doi.dedup.....efb58e5b545a4a8a78ae858415ef2b4f