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Genetic diversity, QoI fungicide resistance, and mating type distribution of Cercospora sojina-Implications for the disease dynamics of frogeye leaf spot on soybean
- Source :
- PLoS ONE, Vol 12, Iss 5, p e0177220 (2017), PLoS ONE
- Publication Year :
- 2017
- Publisher :
- Public Library of Science (PLoS), 2017.
-
Abstract
- Frogeye leaf spot (FLS), caused by Cercospora sojina, causes significant damage to soybean in the U.S. One control strategy is the use of quinone outside inhibitor (QoI) fungicides. QoI resistant isolates were first reported in Tennessee (TN) in 2010. To investigate the disease dynamics of C. sojina, we collected 437 C. sojina isolates in 2015 from Jackson and Milan, TN and used 40 historical isolates collected from 2006-2009 from TN and ten additional states for comparison. A subset of 186 isolates, including historical isolates, were genotyped for 49 single nucleotide polymorphism (SNP) markers and the QoI resistance locus, revealing 35 unique genotypes. The genotypes clustered into three groups with two groups containing only sensitive isolates and the remaining group containing all resistant isolates and a dominant clonal lineage of 130 isolates. All 477 C. sojina isolates were genotyped for the QoI locus revealing 344 resistant and 133 sensitive isolates. All isolates collected prior to 2015 were QoI sensitive. Both mating type alleles (MAT1-1-1 and MAT1-2) were found in Jackson and Milan, TN and recovered from single lesions suggesting sexual recombination may play a role in the epidemiology of field populations. Analysis of C. sojina isolates using SNP markers proved useful to investigate population diversity and to elaborate on diversity as it relates to QoI resistance and mating type.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Leaves
Heredity
Drug Resistance
lcsh:Medicine
Artificial Gene Amplification and Extension
Plant Science
01 natural sciences
Polymerase Chain Reaction
Genotype
lcsh:Science
Fungicides
Genetics
Multidisciplinary
biology
Plant Anatomy
Agriculture
Fungicide
Genetic Mapping
Agrochemicals
Research Article
Pesticide resistance
DNA, Plant
Locus (genetics)
Crops
Variant Genotypes
Mycology
Research and Analysis Methods
Polymorphism, Single Nucleotide
03 medical and health sciences
Ascomycota
Botany
Leaf spot
Fungal Genetics
Molecular Biology Techniques
Molecular Biology
Genetic diversity
Evolutionary Biology
Population Biology
lcsh:R
Genetic Variation
Biology and Life Sciences
biology.organism_classification
Fungicides, Industrial
030104 developmental biology
Genetic marker
Genetic Loci
Cercospora sojina
lcsh:Q
Soybeans
Soybean
Population Genetics
010606 plant biology & botany
Crop Science
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 12
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....efb1646901ae92e372fd89db86f1bf79