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Cancer stem‐like cells with hybrid epithelial/mesenchymal phenotype leading the collective invasion
- Source :
- Cancer Science
- Publication Year :
- 2020
- Publisher :
- Wiley, 2020.
-
Abstract
- Collective invasion of cancer cells is the key process of circulating tumor cell (CTC) cluster formation, and greatly contributes to metastasis. Cancer stem‐like cells (CSC) have a distinct advantage of motility for metastatic dissemination. To verify the role of CSC in the collective invasion, we performed 3D assays to investigate the collective invasion from cancer cell spheroids. The results demonstrated that CSC can significantly promote both collective and single‐cell invasion. Further study showed that CSC prefer to move outside and lead the collective invasion. More interestingly, approximately 60% of the leader CSC in collective invasion co–expressed both epithelial and mesenchymal genes, while only 4% co–expressed in single invasive CSC, indicating that CSC with hybrid epithelial/mesenchymal phenotype play a key role in cancer cell collective invasion.<br />To verify the role of CSC in the collective invasion, we performed 3D assays for investigating the collective invasion from cancer cell spheroids. The results demonstrated that CSC act as leaders to promote collective invasion with hybrid epithelial/mesenchymal phenotype. Our present observations may predict a new strategy for cancer metastasis, including interventions targeting CSC.
- Subjects :
- 0301 basic medicine
Cancer Research
Epithelial-Mesenchymal Transition
single invasion
Motility
Breast Neoplasms
Biology
leader
Metastasis
Mice
03 medical and health sciences
0302 clinical medicine
Circulating tumor cell
Cell, Molecular, and Stem Cell Biology
Cell Movement
Cell Line, Tumor
Spheroids, Cellular
Biomarkers, Tumor
medicine
Animals
Humans
Neoplasm Invasiveness
Neoplasm Metastasis
hybrid epithelial/mesenchymal
collective invasion
Mesenchymal stem cell
Spheroid
Cancer
Original Articles
General Medicine
medicine.disease
Gene Expression Regulation, Neoplastic
030104 developmental biology
Oncology
030220 oncology & carcinogenesis
Cancer cell
MCF-7 Cells
Neoplastic Stem Cells
Cancer research
Original Article
Female
cancer stem‐like cells
Neoplasm Transplantation
Mesenchymal phenotype
Subjects
Details
- ISSN :
- 13497006 and 13479032
- Volume :
- 111
- Database :
- OpenAIRE
- Journal :
- Cancer Science
- Accession number :
- edsair.doi.dedup.....efa8be40735130ceac960a6a7f75b6de