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Rat Epidermal Growth Factors: Purification and Tissue Content
- Source :
- Endocrinologia Japonica. 36:289-297
- Publication Year :
- 1989
- Publisher :
- Japan Endocrine Society, 1989.
-
Abstract
- Rat epidermal growth factor (rEGF) was isolated from the submaxillary gland of male rat by reversed phase high performance liquid chromatography and ion exchange chromatography. The binding of purified rEGF to human carcinoma cells (A-431) and its tritiated thymidine uptake on rat epidermal fibroblast cells (FR) were almost the same as those of purified commercially available mouse EGF (mEGF). Antisera to rEGF was raised in rabbits and a radioimmunoassay (RIA) system was established. The assay range of the RIA was about 1.0 to 100 ng/ml. The within assay coefficients of variation were 5 to 10%, while the between assay coefficients of variation were 5 to 13%. The tissue content of rEGF of male rats (10 weeks old) was examined. As a result, the submaxillary gland was found to contain a very high concentration of rEGF (214 micrograms/g wet tissue) as predicted, and digestive tissues, stomach, intestine and duodenum contained 2.49, 3.57, 9.44 ng/g wet tissue, respectively. The amounts in prostate and seminal vesicle were relatively high, being 65.6 and 2,268 ng/g wet tissue, respectively. The amount in the submaxillary gland increased markedly after 7 weeks of age. These results suggest that EGF is an important factor in gonadal function.
- Subjects :
- Male
Aging
medicine.medical_specialty
Submandibular Gland
Ion chromatography
Radioimmunoassay
High-performance liquid chromatography
Radioligand Assay
chemistry.chemical_compound
Endocrinology
Seminal vesicle
Epidermal growth factor
Internal medicine
medicine
Animals
Fibroblast
Cells, Cultured
Epidermal Growth Factor
Stomach
General Engineering
Rats, Inbred Strains
Rats
medicine.anatomical_structure
chemistry
Thymidine
Subjects
Details
- ISSN :
- 21856370 and 00137219
- Volume :
- 36
- Database :
- OpenAIRE
- Journal :
- Endocrinologia Japonica
- Accession number :
- edsair.doi.dedup.....ef9deec263ebdb2efb3d76c37aeea96b