Back to Search
Start Over
An evaluation of the biocompatibility and osseointegration of novel glass fiber reinforced composite implants: In vitro and in vivo studies
- Source :
- Dental materials : official publication of the Academy of Dental Materials. 34(3)
- Publication Year :
- 2017
-
Abstract
- Objectives The aim of this study was to evaluate the in vitro biocompatibility and in vivo osseointegration of three novel bioactive glass fiber reinforced composite (GFRC) implants and to compare these with metal (Ti6Al4V) implants. Methods The surfaces of these experimental substrates were characterized by scanning electron microscopy (SEM), a 2D profilometer and by contact angle measurement. In vitro biological performance was assessed using MG-63 human osteoblast-like cell morphology, cell proliferation assays and the alkaline phosphatase (ALP) activity testing. Furthermore, in vivo osseointegration performance was examined by installing samples into rabbit femurs and evaluated the results using micro-CT, histology and histomorphometrical analysis; these assessments were carried out after 1, 2, 4 and 8 weeks of healing. Results The results showed that moderate surface roughness, moderate hydrophilic exposure and moderate homogenous exposure of bioactive glass fibers were present for all of the GFRC substrates. Furthermore, MG-63 cells, when cultured on all of the GFRC substrates, grew well and exhibited a more differentiated phenotype than cells grown on titanium alloy (Ti6Al4V) substrate. Histological evaluation revealed more newly-formed bone regeneration within the thread of the GFRC implants during the initial healing period. In addition, the novel GFRC implants with a bioactive Bio-fiber structure and glass particles within the epoxy resin matrix showed better bone volume/tissue volume (BV/TV) values at 4 weeks and this was accompanied by bone-implant contact (BIC) values at 8 weeks comparable to the Ti6Al4V group. Significance These findings demonstrated that novel GFRC implants seem to show improved osteogenesis and osseointegration functionality and have potential as a substitute for Ti6Al4V, or other metal-based materials, when used for clinically dental and orthopedic applications.
- Subjects :
- Male
Materials science
Biocompatibility
Surface Properties
Glass fiber
Biocompatible Materials
02 engineering and technology
Cell morphology
Composite Resins
Osseointegration
law.invention
03 medical and health sciences
Dental Materials
0302 clinical medicine
Implants, Experimental
In vivo
law
Materials Testing
Alloys
Animals
Humans
General Materials Science
Femur
Bone regeneration
General Dentistry
Cell Proliferation
Dental Implants
Titanium
Osteoblasts
Cell Differentiation
030206 dentistry
Epoxy
021001 nanoscience & nanotechnology
Dental Prosthesis Design
Mechanics of Materials
visual_art
Bioactive glass
visual_art.visual_art_medium
Microscopy, Electron, Scanning
Wettability
Glass
Rabbits
0210 nano-technology
Biomedical engineering
Subjects
Details
- ISSN :
- 18790097
- Volume :
- 34
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Dental materials : official publication of the Academy of Dental Materials
- Accession number :
- edsair.doi.dedup.....ef953001ce9075f931a1efb9519cb342