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Cutaneous Immunoprofiles of Three Spotted Fever Group Rickettsia Cases
- Source :
- Infection and Immunity
- Publication Year :
- 2020
- Publisher :
- American Society for Microbiology, 2020.
-
Abstract
- Spotted fever group rickettsia (SFGR) can cause mild to fatal illness. The early interaction between the host and rickettsia in skin is largely unknown, and the pathogenesis of severe rickettsiosis remains an important topic. A surveillance of SFGR infection by PCR of blood and skin biopsy specimens followed by sequencing and immunohistochemical (IHC) detection was performed on patients with a recent tick bite between 2013 and 2016. Humoral and cutaneous immunoprofiles were evaluated in different SFGR cases by serum cytokine and chemokine detection, skin IHC staining, and transcriptome sequencing (RNA-seq).<br />Spotted fever group rickettsia (SFGR) can cause mild to fatal illness. The early interaction between the host and rickettsia in skin is largely unknown, and the pathogenesis of severe rickettsiosis remains an important topic. A surveillance of SFGR infection by PCR of blood and skin biopsy specimens followed by sequencing and immunohistochemical (IHC) detection was performed on patients with a recent tick bite between 2013 and 2016. Humoral and cutaneous immunoprofiles were evaluated in different SFGR cases by serum cytokine and chemokine detection, skin IHC staining, and transcriptome sequencing (RNA-seq). A total of 111 SFGR cases were identified, including 79 “Candidatus Rickettsia tarasevichiae,” 22 Rickettsia raoultii, 8 Rickettsia sibirica, and 2 Rickettsia heilongjiangensis cases. The sensitivity to detect SFGR in skin biopsy specimens (9/24, 37.5%) was significantly higher than that in blood samples (105/2,671, 3.9%) (P
- Subjects :
- 0301 basic medicine
Biopsy
030106 microbiology
Immunology
ved/biology.organism_classification_rank.species
skin biopsy specimens
rickettsiosis
Tick
Microbiology
03 medical and health sciences
Rickettsia heilongjiangensis
medicine
Humans
Immunologic Factors
Rickettsia
Skin
Host Response and Inflammation
Tick Bites
biology
medicine.diagnostic_test
ved/biology
pathogenesis
Gene Expression Profiling
Rickettsia sibirica
Spotted Fever Group Rickettsiosis
bacterial infections and mycoses
biology.organism_classification
medicine.disease
Virology
Immunohistochemistry
Spotted fever
030104 developmental biology
Infectious Diseases
Rickettsiosis
Skin biopsy
Cytokines
Parasitology
transcriptome
Subjects
Details
- Language :
- English
- ISSN :
- 10985522 and 00199567
- Volume :
- 88
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Infection and Immunity
- Accession number :
- edsair.doi.dedup.....ef914eb4d51f747b15bdea9e993ac0c2