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Neural Stem Cell Transplant Survival in Brains of Mice: Assessing the Effect of Immunity and Ischemia by using Real-time Bioluminescent Imaging
- Source :
- Radiology. 241:822-830
- Publication Year :
- 2006
- Publisher :
- Radiological Society of North America (RSNA), 2006.
-
Abstract
- To use bioluminescent imaging in a murine transplant model to monitor the in vivo responses of transplanted luciferase-gene-positive neural progenitor cells (NPCs) to host immunity and ischemia.All animal studies were conducted according to institutional guidelines, with approval of the Subcommittee on Research Animal Care. Cranial windows were created in all animals, and all animals underwent NPC (C17.2-Luc-GFP-gal) transplantation into the right basal ganglia. An observational study was performed on C57 BL/6 (n = 5), nude (n = 4), and CD-1 (n = 4) mice, with bioluminescent imaging performed at days 7, 11, and 14 after transplantation. A study on the effects of ischemia was performed in a similar manner, but with the following differences: On day 9 after transplantation, the C57 BL/6 mice underwent 18 minutes of transient forebrain ischemia by means of temporary bilateral carotid occlusions (n = 6). A control group of C57 BL/6 mice underwent sham surgery (n = 6). Bioluminescent imaging was performed on the ischemic animals and control animals at days 7, 9, 11, and 14. Repeated-measures analysis of variance or Student t test was used to compare the means of the luciferase activities.In vivo cell tracking demonstrated that (a) C17.2-Luc-GFP-gal NPCs survived and proliferated better in the T-cell deficient nude mice than in the immunocompetent C57 BL/6 or CD-1 mice, in which progressive immune mediated cell loss was shown, and (b) transient forebrain ischemia appeared, unexpectedly, to act as a short-term stimulus to transplanted NPC growth and survival in immunocompetent mice.Immune status and host immunity can have an influence on NPC graft survival, and these changes can be noninvasively assessed with bioluminescent imaging in this experimental model.
- Subjects :
- Pathology
medicine.medical_specialty
Ischemia
Mice, Nude
Basal Ganglia
Brain Ischemia
Mice
In vivo
Immunity
Cell Line, Tumor
medicine
Animals
Radiology, Nuclear Medicine and imaging
Luciferases
Cell Proliferation
business.industry
Stem Cells
Graft Survival
Sham surgery
Flow Cytometry
medicine.disease
Neural stem cell
Mice, Inbred C57BL
Transplantation
Luminescent Measurements
Animal studies
Stem cell
business
Stem Cell Transplantation
Subjects
Details
- ISSN :
- 15271315 and 00338419
- Volume :
- 241
- Database :
- OpenAIRE
- Journal :
- Radiology
- Accession number :
- edsair.doi.dedup.....ef62c641f66a62a4a1ee78019728947e