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4-1BB Protects Dendritic Cells from Prostate Cancer-Induced Apoptosis
- Source :
- Pathology & Oncology Research. 19:177-181
- Publication Year :
- 2012
- Publisher :
- Springer Science and Business Media LLC, 2012.
-
Abstract
- It has been shown that human prostate cancer (PCa) cells induced apoptotic death of the most potent antigen-presenting cells, dendritic cells (DCs), which are responsible for the induction of specific antitumor immune responses. Here, we investigated the function of 4-1BB on protecting DCs from prostate cancer-induced apoptosis with an agonistic mAb to 4-1BB. RM-1 cells and DCs were co-incubated for 48 h and DC apoptosis was assessed by Annexin Vassay. TNF-α and IL-12 production were assessed by enzyme-linked immunosorbent assay (ELISA) and Bcl-2 and Bcl-xL on DCs were analyzed by Western blot. We have shown that co-incubation of RM-1 cells with DCs is accompanied by an increased level of DCs apoptosis. Triggering 4-1BB on DCs resulted in increased resistance of DCs to RM-1 cells-induced apoptosis, which was owing to the up-regulated expression of Bcl-2 and Bcl-xL, and increased secretion of TNF-αand IL-12. These results demonstrate that triggering 4-1BB on DCs could increased resistance of DCs to PCa-induced apoptosis.
- Subjects :
- Male
Cancer Research
medicine.drug_class
bcl-X Protein
Apoptosis
chemical and pharmacologic phenomena
Biology
Monoclonal antibody
Pathology and Forensic Medicine
Mice
Prostate cancer
Immune system
Western blot
Annexin
Cell Line, Tumor
medicine
Animals
Secretion
Follicular dendritic cells
medicine.diagnostic_test
Tumor Necrosis Factor-alpha
Antibodies, Monoclonal
Prostatic Neoplasms
hemic and immune systems
Dendritic Cells
General Medicine
medicine.disease
Interleukin-12
Up-Regulation
Cell biology
Mice, Inbred C57BL
4-1BB Ligand
Proto-Oncogene Proteins c-bcl-2
Oncology
Female
Subjects
Details
- ISSN :
- 15322807 and 12194956
- Volume :
- 19
- Database :
- OpenAIRE
- Journal :
- Pathology & Oncology Research
- Accession number :
- edsair.doi.dedup.....ef5e30f5ae32d12dc37f51bc05f7aced