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Transcriptomic Profiling of the Allorecognition Response to Grafting in the Demosponge Amphimedon queenslandica
- Source :
- Marine Drugs; Volume 15; Issue 5; Pages: 136, Marine Drugs, Vol 15, Iss 5, p 136 (2017), Marine Drugs
- Publication Year :
- 2017
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2017.
-
Abstract
- Sponges, despite their simple body plan, discriminate between self and nonself with remarkable specificity. Sponge grafting experiments simulate the effects of natural self or nonself contact under laboratory conditions. Here we take a transcriptomic approach to investigate the temporal response to self and nonself grafts in the marine demosponge Amphimedon queenslandica. Auto- and allografts were established, observed and sampled over a period of three days, over which time the grafts either rejected or accepted, depending on the identity of the paired individuals, in a replicable and predictable manner. Fourteen transcriptomes were generated that spanned the auto- and allograft responses. Self grafts fuse completely in under three days, and the process appears to be controlled by relatively few genes. In contrast, nonself grafting results in a complete lack of fusion after three days, and appears to involve a broad downregulation of normal biological processes, rather than the mounting of an intense defensive response.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Pharmaceutical Science
RNA-Seq
chemical and pharmacologic phenomena
010603 evolutionary biology
01 natural sciences
Article
Transcriptome
sponge
03 medical and health sciences
allorecognition
Demosponge
Drug Discovery
Animals
histocompatibility
invertebrates
Porifera
self-nonself recognition
Allorecognition
Pharmacology, Toxicology and Pharmaceutics (miscellaneous)
lcsh:QH301-705.5
biology
Gene Expression Profiling
Anatomy
biology.organism_classification
Amphimedon queenslandica
Cell biology
Sponge
030104 developmental biology
Body plan
surgical procedures, operative
Gene Expression Regulation
lcsh:Biology (General)
Subjects
Details
- Language :
- English
- ISSN :
- 16603397
- Database :
- OpenAIRE
- Journal :
- Marine Drugs; Volume 15; Issue 5; Pages: 136
- Accession number :
- edsair.doi.dedup.....ef56c90db2ed36cb6b2f4c2c90378386
- Full Text :
- https://doi.org/10.3390/md15050136