Back to Search
Start Over
Detection and Specific Enumeration of Multi-Strain Probiotics in the Lumen Contents and Mucus Layers of the Rat Intestine After Oral Administration
- Source :
- Probiotics and antimicrobial proteins. 1(2)
- Publication Year :
- 2009
-
Abstract
- Although the detection of viable probiotic bacteria following their ingestion and passage through the gastrointestinal tract (GIT) has been well documented, their mucosal attachment in vivo is more difficult to assess. In this study, we investigated the survival and mucosal attachment of multi-strain probiotics transiting the rat GIT. Rats were administered a commercial mixture of the intestinal probiotics Lactobacillus acidophilus LA742, Lactobacillus rhamnosus L2H and Bifidobacterium lactis HN019 and the oral probiotic Streptococcus salivarius K12 every 12 h for 3 days. Intestinal contents, mucus and faeces were tested 6 h, 3 days and 7 days after the last dose by strain-specific enumeration on selective media and by denaturing gradient gel electrophoresis. At 6 h, viable cells and DNA corresponding to all four probiotics were detected in the faeces and in both the lumen contents and mucus layers of the ileum and colon. Viable probiotic cells of B. lactis and L. rhamnosus were detected for 7 days and L. acidophilus for 3 days after the last dose. B. lactis and L. rhamnosus persisted in the ileal mucus and colon contents, whereas the retention of L. acidophilus appeared to be relatively higher in colonic mucus. No viable cells of S. salivarius K12 were detected in any of the samples at either day 3 or 7. The study demonstrates that probiotic strains of intestinal origin but not of oral origin exhibit temporary colonisation of the rat GIT and that these strains may have differing relative affinities for colonic and ileal mucosa.
- Subjects :
- Gastrointestinal tract
biology
food and beverages
Ileum
biology.organism_classification
Microbiology
Mucus
law.invention
Probiotic
fluids and secretions
medicine.anatomical_structure
Lactobacillus acidophilus
Streptococcus salivarius
Lactobacillus rhamnosus
law
medicine
Molecular Medicine
Ingestion
Molecular Biology
Subjects
Details
- ISSN :
- 18671306
- Volume :
- 1
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Probiotics and antimicrobial proteins
- Accession number :
- edsair.doi.dedup.....ef523729c6c7ec004b80bf5619f4ae0d