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Prophylactic effects of secretion metabolites of dairy lactobacilli through downregulation of ErbB-2 and ErbB-3 genes on colon cancer cells
Prophylactic effects of secretion metabolites of dairy lactobacilli through downregulation of ErbB-2 and ErbB-3 genes on colon cancer cells
- Source :
- European Journal of Cancer Prevention. 29:201-209
- Publication Year :
- 2020
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 2020.
-
Abstract
- Colon cancer is one of the most prevalent cancers, and intestinal microbial community plays a pivotal role in colorectal tumor genesis. Probiotics as live microorganisms may be able to exert an anticancer effect in colon cancer. The aim of this study was to isolate and identify Lactobacillus spp. from traditional dairy products with probiotic properties and to investigate their anticancer effects through ErbB-2 and ErbB-3 gene expression in colon cancer cells. The isolated lactobacilli from yogurt and cheese samples were molecularly identified by blasting of 16-23s rDNA region PCR sequenced products. The probiotic properties, including acid and bile tolerance, antimicrobial activity, and antibiotic susceptibility, were assayed. The proliferation inhibition effects of lactobacilli secretion metabolites with probiotic potential on colon cancer cell lines (HT-29 and caco-2) were analyzed using MTT assay. The real-time PCR was used for assessment of ErbB-2 and ErbB-3 gene expression after being treated with probiotics. Four species of bacteria with the most probiotic properties, including Lactobacillus casei, Lactobacillus paracasei, Lactobacillus rhamnosus, and Lactobacillus plantarum, were characterized and their effects on different human cell lines were taken into consideration. Total bacterial secretions significantly reduced the viability of HT-29 and caco-2 cancer cells compared with untreated controls. The metabolites secreted by bacteria downregulated the expression of ErbB-2 and ErbB-3 genes in colon cancer cells. The present study indicated that probiotic bacteria isolated from traditional dairy products exert anticancer effect on colon cancer cells through the downregulation of ErbB-2 and ErbB-3 gene expression.
- Subjects :
- 0301 basic medicine
Cancer Research
Lactobacillus casei
Receptor, ErbB-3
Lactobacillus paracasei
Cultured Milk Products
Receptor, ErbB-2
Epidemiology
Colorectal cancer
Down-Regulation
Iran
Microbiology
law.invention
03 medical and health sciences
Probiotic
0302 clinical medicine
Lactobacillus rhamnosus
law
Lactobacillus
medicine
Humans
biology
Probiotics
Public Health, Environmental and Occupational Health
food and beverages
biology.organism_classification
medicine.disease
Culture Media
Gene Expression Regulation, Neoplastic
HEK293 Cells
030104 developmental biology
Oncology
030220 oncology & carcinogenesis
Colonic Neoplasms
Immunology
Cancer cell
Caco-2 Cells
Lactobacillus plantarum
Subjects
Details
- ISSN :
- 09598278
- Volume :
- 29
- Database :
- OpenAIRE
- Journal :
- European Journal of Cancer Prevention
- Accession number :
- edsair.doi.dedup.....ef4c5ffa57334451f9a223858182f465
- Full Text :
- https://doi.org/10.1097/cej.0000000000000393