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Experimental and theoretical study of the interaction of single-stranded DNA homopolymers and a monomethine cyanine dye: nature of specific binding
- Source :
- Photochemical & Photobiological Sciences. 4:798-802
- Publication Year :
- 2005
- Publisher :
- Springer Science and Business Media LLC, 2005.
-
Abstract
- The photophysical properties of an intercalating unsymmetrical monomethine cyanine dye and single-stranded DNA homopolymers show strong association for poly(dA) and poly(dG), but not for poly(dC) and poly(dT), as determined by several spectroscopic techniques and molecular dynamics calculations. While poly(dA) and poly(dG) appear to bind the dye as a monomer (with dramatic increase in fluorescence), poly(dC) and poly(dT) bind only very weakly, and seem to promote dye aggregation. Only in the case of poly(dA) there seems to be a unique, well defined form of intercalation, that molecular dynamics calculations suggest involve the quinoline ring between two bases, in an arrangement that should favor pi-stacking; consistently with this, the decay of the fluorescence shows a single exponential, the absorption spectrum shows a shift in the dye maximum, the fluorescence is strong, and the induced circular dichroism follows a simple pattern.
- Subjects :
- Models, Molecular
Circular dichroism
Absorption spectroscopy
Circular Dichroism
Quinoline
Intercalation (chemistry)
DNA, Single-Stranded
Carbocyanines
Photochemistry
Fluorescence
Intercalating Agents
chemistry.chemical_compound
Molecular dynamics
Spectrometry, Fluorescence
Monomer
chemistry
Physical and Theoretical Chemistry
Cyanine
Subjects
Details
- ISSN :
- 14749092 and 1474905X
- Volume :
- 4
- Database :
- OpenAIRE
- Journal :
- Photochemical & Photobiological Sciences
- Accession number :
- edsair.doi.dedup.....ef465c364e110a50502b96fcff3ac868