Back to Search
Start Over
Iron and exercise induced alterations in antioxidant status. Protection by dietary milk proteins
- Source :
- Free Radical Research, Free Radical Research, Taylor & Francis, 2006, 40 (5), pp.535-542. ⟨10.1080/10715760500451202⟩
- Publication Year :
- 2006
- Publisher :
- HAL CCSD, 2006.
-
Abstract
- Lipid peroxidation stress induced by iron supplementation can contribute to the induction of gut lesions. Intensive sports lead to ischemia reperfusion, which increases free radical production. Athletes frequently use heavy iron supplementation, whose effects are unknown. On the other hand, milk proteins have in vitro antioxidant properties, which could counteract these potential side effects. The main aims of the study were: (1) to demonstrate the effects of combined exercise training (ET) and iron overload on antioxidant status; (2) to assess the protective properties of casein in vivo; (3) to study the mechanisms involved in an in vitro model. Antioxidant status was assessed by measuring the activity of antioxidant enzymes (superoxide dismutase (SOD); glutathione peroxidase (GSH-Px)), and on the onset of aberrant crypts (AC) in colon, which can be induced by lipid peroxidation. At day 30, all ET animals showed an increase in the activity of antioxidant enzymes, in iron concentration in colon mucosa and liver and in the number of AC compared to untrained rats. It was found that Casein's milk protein supplementation significantly reduced these parameters. Additional information on protective effect of casein was provided by measuring the extent of TBARS formation during iron/ascorbate-induced oxidation of liposomes. Free casein and casein bound to iron were found to significantly reduce iron-induced lipid peroxidation. The results of the overall study suggest that Iron supplementation during intensive sport training would decrease anti-oxidant status. Dietary milk protein supplementation could at least partly prevent occurrence of deleterious effects to tissue induced by iron overload.
- Subjects :
- Antioxidant
030309 nutrition & dietetics
medicine.medical_treatment
Biochemistry
PEROXIDATION
fer
Lipid peroxidation
Rats, Sprague-Dawley
chemistry.chemical_compound
0302 clinical medicine
iron
protéine du lait
Casein
IRON OVERLOAD
2. Zero hunger
chemistry.chemical_classification
0303 health sciences
biology
Chemistry
Glutathione peroxidase
Caseins
General Medicine
3. Good health
Liver
protéine
030220 oncology & carcinogenesis
medicine.medical_specialty
Colon
oligoélément
Thiobarbituric Acid Reactive Substances
Superoxide dismutase
03 medical and health sciences
antioxydant
In vivo
milk protein
Internal medicine
Physical Conditioning, Animal
medicine
TBARS
Animals
EXERCICE
Glutathione Peroxidase
Superoxide Dismutase
Diet
Rats
Endocrinology
Enzyme
biology.protein
RAT
Lipid Peroxidation
protein
[CHIM.OTHE]Chemical Sciences/Other
Subjects
Details
- Language :
- English
- ISSN :
- 10715762 and 10292470
- Database :
- OpenAIRE
- Journal :
- Free Radical Research, Free Radical Research, Taylor & Francis, 2006, 40 (5), pp.535-542. ⟨10.1080/10715760500451202⟩
- Accession number :
- edsair.doi.dedup.....ef3c679c75ab33b9b0fc5d55282a99dd