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Overview of the in vitro stability of commonly measured vitamins and carotenoids in whole blood
- Source :
- Annals of Clinical Biochemistry, Annals of Clinical Biochemistry, SAGE Publications, 2015, 52 (2), pp.259-269. ⟨10.1177/0004563214542471⟩
- Publication Year :
- 2015
- Publisher :
- HAL CCSD, 2015.
-
Abstract
- Background The pre-analytical stabilities of vitamins A, E, K, B1, B2, B6, B12, C, carotenoids and folates in whole blood were studied. The aim of this work was to provide clear and workable pre-analytical procedures specifying optimal delay before freezing for laboratories which perform themselves such analyses or which receive and transfer such specimens to referral laboratories. Methods The stability of vitamins was studied in whole blood at room temperature after light exposure up to 24 h (vitamin C), 48 h (vitamins A, E, B1, B2, B6 and carotenoids) and 72 h (vitamins K, B12, red blood cell (RBC) and serum folates). Vitamin C stability after baseline acidification was analysed up to 48 h. Changes observed were compared to a clinical cut-off defined as total change limit based on a combination of analytical performance and within-subject variation. Results Clinically and statistically significant changes occurred only in vitamins C (−22.5%), B6 (+9.9%) and serum folates (−16.8%) concentrations after 6, 24 and 48 h, respectively. Vitamins A, E, K, B1, B2, B12, RBC folates and carotenoids showed good stability up to 48 or 72 h. Vitamin C in acidified serum conserved at room temperature appeared unstable. The optimal condition for acidified vitamin C conservation was at less than −20℃. Conclusion The majority of vitamins remain stable for up to 48 h. Vitamin C quantification requires serum acidification followed by freezing as soon as possible. Freezing, respectively, within 12 h and 24 h for determination of plasma vitamin B6 and serum folates concentrations is recommended.
- Subjects :
- Male
Serum
030213 general clinical medicine
Time Factors
Light
Clinical Biochemistry
030204 cardiovascular system & hematology
Plasma
0302 clinical medicine
contrôle de la qualité
vitamine
Carotenoid
Light exposure
Whole blood
chemistry.chemical_classification
basse température
hematology
Vitamins
General Medicine
Hydrogen-Ion Concentration
Middle Aged
carotenoid
Cold Temperature
medicine.anatomical_structure
vitamin m
nutrition
chromatography
Female
[SDV.IB]Life Sciences [q-bio]/Bioengineering
France
Adult
Quality Control
low temperature
Young Adult
03 medical and health sciences
carotenoide
Folic Acid
medicine
sang
Humans
quality assurance and control
Chromatography
Vitamin C
Reproducibility of Results
Carotenoids
In vitro
Red blood cell
chemistry
Chemistry, Clinical
acide folique
reproductibilité de la méthode
Subjects
Details
- Language :
- English
- ISSN :
- 00045632
- Database :
- OpenAIRE
- Journal :
- Annals of Clinical Biochemistry, Annals of Clinical Biochemistry, SAGE Publications, 2015, 52 (2), pp.259-269. ⟨10.1177/0004563214542471⟩
- Accession number :
- edsair.doi.dedup.....ef333507640c6859178e7ccaa26ea045