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Opposite effects of 5-hydroxymethyluracil on mitogenic response of T cells stimulated through T-cell receptor or through T-cell receptor and CD28 co-receptor molecule
- Source :
- Bulletin of experimental biology and medicine. 150(1)
- Publication Year :
- 2010
-
Abstract
- The effect of a synthetic analog of “minor” pyrimidine base 5-hydroxymethyluracil (0.01- 10 μg/ml) on the mitogenic response of donor peripheral blood T cells was studied in vitro under conditions of lymphocyte stimulation through T-cell receptor (antiCD3 monoclonal antibodies) or through T-cell receptor and CD28 co-receptor molecule (antiCD3 and antiCD28 monoclonal antibodies). 5-Hydroxymethyluracil suppressed the mitogenic response of T cells stimulated with antiCD3 antibodies, which was paralleled by an increase in the count of silent cells and decrease in the count of dividing lymphocytes, but not by stimulation of apoptosis of activated cells. Under conditions of integration of stimuli (lymphocyte stimulation with antiCD3 and antiCD28), 5-hydroxymethyluracil stimulated the mitogenic response, which was paralleled by suppression of activation apoptosis and increase in proliferative potential of cells.
- Subjects :
- Co-receptor
biology
medicine.drug_class
T-Lymphocytes
T-cell receptor
Receptors, Antigen, T-Cell
CD28
Stimulation
General Medicine
Monoclonal antibody
Lymphocyte Activation
Molecular biology
General Biochemistry, Genetics and Molecular Biology
Pentoxyl
Adjuvants, Immunologic
CD28 Antigens
biology.protein
medicine
Humans
IL-2 receptor
Antibody
Receptor
Cells, Cultured
Subjects
Details
- ISSN :
- 15738221
- Volume :
- 150
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Bulletin of experimental biology and medicine
- Accession number :
- edsair.doi.dedup.....ef23bb47033a89a8205b067046611390