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Flavonoid epimers from custard apple leaves, a rapid screening and separation by HSCCC and their antioxidant and hypoglycaemic activities evaluation
- Source :
- Scientific Reports, Vol 10, Iss 1, Pp 1-11 (2020), Scientific Reports
- Publication Year :
- 2020
- Publisher :
- Nature Publishing Group, 2020.
-
Abstract
- Leaves of custard apple are widely used in many places as a popular dietary supplement for the treatment of diabetes. Flavonoids are known to have anti-diabetic activity. In this study, the main flavonoid epimers were separated. The crude extract was first screened by HPLC-DAD before and after incubation with DPPH method to evaluate the antioxidants. An efficient extraction method was employed to remove non-flavonoid components. Subsequently, five main flavonoids with two pairs of epimers including quercetin-3-O-robinobioside, rutin, quercetin-3-O-β-D-glucoside, kaempferol-3-O-robinobioside, and kaempferol-3-O-rutinoside were successfully separated by high-speed counter-current chromatography with ethyl acetate/n-butanol/water (4:1:5, v/v) coupled with online-storage inner-recycling mode. The structures of the separated compounds were identified by spectral techniques. The purity of the separated flavonoid glycosides was over 98%, as determined by HPLC. The separated pure constituents were found to possess the antioxidant capacities following DPPH radical scavenging protocol. The compounds (1-3) exhibited better antioxidant activity. Furthermore, the glucose uptake of crude flavonoid extract had better results than the crude ethanol extract. The present study demonstrates that the efficacy of custard apple leaves in lowering glucose level, and antioxidant capacities of separated pure compounds probably appear to be predominantly responsible for hypoglycaemic properties on HepG2 cells.
- Subjects :
- food.ingredient
Antioxidant
DPPH
medicine.medical_treatment
Flavonoid
Drug Evaluation, Preclinical
Ethyl acetate
lcsh:Medicine
Medicinal chemistry
01 natural sciences
High-performance liquid chromatography
Article
Annona
Antioxidants
Techniques and instrumentation
Rutin
chemistry.chemical_compound
food
Picrates
medicine
Humans
Hypoglycemic Agents
Insulin
heterocyclic compounds
lcsh:Science
Countercurrent Distribution
Chromatography, High Pressure Liquid
Flavonoids
chemistry.chemical_classification
Multidisciplinary
Chromatography
Molecular Structure
Plant Extracts
010405 organic chemistry
Biphenyl Compounds
010401 analytical chemistry
lcsh:R
food and beverages
Stereoisomerism
Hep G2 Cells
Custard-apple
Metformin
0104 chemical sciences
Plant Leaves
Biphenyl compound
Glucose
chemistry
Solvents
lcsh:Q
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 10
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....ef176e3ef48e72144495fe908a88af7c