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Detection and molecular characterisation of adenovirus in children under 5 years old with diarrhoea
- Source :
- Volume: 47, Issue: 5 1463-1471, Turkish Journal of Medical Sciences
- Publication Year :
- 2017
- Publisher :
- The Scientific and Technological Research Council of Turkey (TUBITAK-ULAKBIM) - DIGITAL COMMONS JOURNALS, 2017.
-
Abstract
- Background/aim: To determine the frequency, genotype distribution, and genetic relatedness of adenoviruses in children under 5 years old with diarrhoea and to investigate their distribution according to clinical findings, age, months, and seasons. Materials and methods: Stool samples were collected from 180 children with acute gastroenteritis who presented from July 2007 through June 2011 at the Ankara Training and Education Hospital. Stool samples were analysed by immune chromatographic test (ICT), enzyme immunoassay (EIA), and polymerase chain reaction (PCR). All adenovirus types were determined by nucleotide sequence analysis. A phylogenetic tree was constructed by Mega 6.0 using the neighbour-joining method. Results: Five percent of the samples were positive for adenovirus (9/180) by ICT, 6.1% (11/180) by EIA, and 13.9% (25/180) by PCR. Adenovirus gastroenteritis did not show any differences in age group, sex, month, or season. In this study, 16 (64%) of the PCR positive samples were AdV41, 6 (24%) were AdV40, 2 (8%) were AdV31, and 1 (4%) was AdV7, as determined by nucleotide sequencing. Conclusion: AdV31 and AdV7 were associated with gastroenteritis. Adenovirus serotypes showed a similarity of 80% (20/25) and 20% (5/25) with Asian and American serotypes, respectively.
- Subjects :
- 0301 basic medicine
Serotype
medicine.diagnostic_test
business.industry
030106 microbiology
Adenovirus gastroenteritis
Nucleotide sequencing
Adenovirus,children,PCR,gastroenteritis,genotype,diarrhoea,phylogenetic analysis
General Medicine
Acute gastroenteritis
Virology
law.invention
03 medical and health sciences
0302 clinical medicine
law
Immunoassay
Genotype
medicine
030212 general & internal medicine
Genetic relatedness
business
Polymerase chain reaction
Subjects
Details
- ISSN :
- 13036165 and 13000144
- Volume :
- 47
- Database :
- OpenAIRE
- Journal :
- TURKISH JOURNAL OF MEDICAL SCIENCES
- Accession number :
- edsair.doi.dedup.....ef113a7885d3c6abd402739e3d0fed58