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Membrane lipid rafts are disturbed in the response of rat skeletal muscle to short-term disuse
- Source :
- Petrov, A M, Kravtsova, V, Matchkov, V, Vasiliev, A N, Zefirov, A L, Chibalin, A V, Heiny, J A & Krivoi, II 2017, ' Membrane lipid rafts disturbance in the response of rat skeletal muscle to short-term disuse. ', American Journal of Physiology: Cell Physiology, vol. 5, no. 312, pp. C627-637 . https://doi.org/10.1152/ajpcell.00365.2016
- Publication Year :
- 2017
- Publisher :
- American Physiological Society, 2017.
-
Abstract
- Marked loss of skeletal muscle mass occurs under various conditions of disuse, but the molecular and cellular mechanisms leading to atrophy are not completely understood. We investigate early molecular events that might play a role in skeletal muscle remodeling during mechanical unloading (disuse). The effects of acute (6–12 h) hindlimb suspension on the soleus muscles from adult rats were examined. The integrity of plasma membrane lipid rafts was tested utilizing cholera toxin B subunit or fluorescent sterols. In addition, resting intracellular Ca2+level was analyzed. Acute disuse disturbed the plasma membrane lipid-ordered phase throughout the sarcolemma and was more pronounced in junctional membrane regions. Ouabain (1 µM), which specifically inhibits the Na-K-ATPase α2 isozyme in rodent skeletal muscles, produced similar lipid raft changes in control muscles but was ineffective in suspended muscles, which showed an initial loss of α2 Na-K-ATPase activity. Lipid rafts were able to recover with cholesterol supplementation, suggesting that disturbance results from cholesterol loss. Repetitive nerve stimulation also restores lipid rafts, specifically in the junctional sarcolemma region. Disuse locally lowered the resting intracellular Ca2+concentration only near the neuromuscular junction of muscle fibers. Our results provide evidence to suggest that the ordering of lipid rafts strongly depends on motor nerve input and may involve interactions with the α2 Na-K-ATPase. Lipid raft disturbance, accompanied by intracellular Ca2+dysregulation, is among the earliest remodeling events induced by skeletal muscle disuse.
- Subjects :
- 0301 basic medicine
Male
Physiology
Biology
Calcium in biology
03 medical and health sciences
chemistry.chemical_compound
Atrophy
Membrane Microdomains
medicine
Animals
Calcium Signaling
Rats, Wistar
Muscle, Skeletal
Lipid raft
Cholesterol
Skeletal muscle
Cell Biology
Hindlimb Suspension
medicine.disease
Skeletal muscle mass
Muscular Disorders, Atrophic
Cell biology
Rats
030104 developmental biology
Membrane
medicine.anatomical_structure
chemistry
Biochemistry
lipids (amino acids, peptides, and proteins)
Calcium
Research Article
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Petrov, A M, Kravtsova, V, Matchkov, V, Vasiliev, A N, Zefirov, A L, Chibalin, A V, Heiny, J A & Krivoi, II 2017, ' Membrane lipid rafts disturbance in the response of rat skeletal muscle to short-term disuse. ', American Journal of Physiology: Cell Physiology, vol. 5, no. 312, pp. C627-637 . https://doi.org/10.1152/ajpcell.00365.2016
- Accession number :
- edsair.doi.dedup.....ef039141695e718e059302a6c5a5a1e7