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Microdialysis sampling combined with ultra-high-performance liquid chromatography-tandem mass spectrometry for the determination of geniposide in dialysate of joint cavities in adjuvant arthritis rats
- Source :
- Rapid communications in mass spectrometry : RCM.
- Publication Year :
- 2017
-
Abstract
- Rationale Microdialysis has been used to detect the concentrations of drugs in tissues. Geniposide (GE), an iridoid glycoside compound, is the main bioactive component of Gardenia jasminoides Ellis fruit. We previously demonstrated that GE could control the activity of cytokines and reduce levels of inflammation in adjuvant arthritis (AA) rats, but the topic of concentration changes over time in the joint synovia of AA is scarcely studied. Methods In this study, microdialysis technique combined with ultra-high-performance liquid chromatography-electrospray ionization coupled with tandem mass spectrometry (UHPLC-ESI-MS/MS) was set up and confirmed to assay GE in the dialysate of the joint cavity in AA rats. Mass detection was conducted in multiple reaction monitoring (MRM) mode with negative electro-spray ionization, and Paeoniflorin (Pae) was used as an internal standard (IS). Results A lower limit of quantitation (LLOQ) of 5 ng/mL was found in this method and with good linearity in the range of 5-4000 ng/mL. All the validation data including accuracy, precision, intra and inter-day repeatability and stability meet the requirements. The relative recoveries of GE were determined at approximately 40.01%. Conclusions The measurements based on microdialysis combined with UHPLC-ESI-MS/MS provide a method for sampling and rapid sensitive analysis of GE in dialysate of joint cavity in AA rats. This method should be considered for future pharmacokinetics studies.
- Subjects :
- 0301 basic medicine
Microdialysis
Chromatography
biology
010401 analytical chemistry
Organic Chemistry
Selected reaction monitoring
Repeatability
Gardenia jasminoides
Paeoniflorin
biology.organism_classification
Tandem mass spectrometry
01 natural sciences
0104 chemical sciences
Analytical Chemistry
03 medical and health sciences
chemistry.chemical_compound
030104 developmental biology
chemistry
Pharmacokinetics
Liquid chromatography–mass spectrometry
Spectroscopy
Subjects
Details
- ISSN :
- 10970231
- Database :
- OpenAIRE
- Journal :
- Rapid communications in mass spectrometry : RCM
- Accession number :
- edsair.doi.dedup.....eef6b1f8d32a6aaa775eff482cdb17ef