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Biofilm constructing variants of Paraburkholderia phytofirmans PsJN outcompete the wild-type form in free-living and static conditions but not in planta
- Source :
- Applied and Environmental Microbiology, Applied and Environmental Microbiology, 2019, 85 (11), pp.UNSP e02670--18. ⟨10.1128/AEM.02670-18⟩, Applied and Environmental Microbiology, American Society for Microbiology, 2019, 85 (11), ⟨10.1128/AEM.02670-18⟩
- Publication Year :
- 2019
- Publisher :
- HAL CCSD, 2019.
-
Abstract
- Members of the genus Burkholderia colonize diverse ecological niches. Among the plant-associated strains, Paraburkholderia phytofirmans PsJN is an endophyte with a broad host range. In spatially structured environment (unshaken broth cultures), biofilm-constructing specialists of P. phytofirmans PsJN colonizing the air-liquid interface arose at high frequency. In addition to forming a robust biofilm in vitro and in planta on Arabidopsis roots, those mucoid phenotypic variants display a reduced swimming ability and modulate the expression of several Microbe Associated Molecular Pattern (MAMPs) including exopolysaccharides (EPS), flagellin and GroEL. Interestingly, the variants induce a low PR1 and PDF1.2 expression compared to the parental strain, suggesting a possible evasion of plant host immunity. We further demonstrated that switching from the planktonic to the sessile form did not involve quorum-sensing genes but arose from spontaneous mutations in two genes belonging to an Iron-Sulfur Cluster: hscA (encoding a co-chaperone protein) and iscS (encoding a cysteine desulfurase). A mutational approach validated the implication of these two genes in the appearance of variants. We showed for the first time that in heterogeneous environment, P. phytofirmans strain PsJN is able to rapidly diversify and co-express a variant that outcompete the wild-type form in free-living and static conditions but not in planta . IMPORTANCE Paraburkholderia phytofirmans strain PsJN is a well-studied plant-associated bacterium known to induce resistance against biotic and abiotic stresses. In this work, we described the spontaneaous appearance of mucoid variants in PsJN from static cultures. We showed that the conversion from wild-type (WT) form to variants (V) correlates with an overproduction of EPS, an enhanced ability to form biofilm in vitro and in planta and a reduced swimming motility. Our results revealed also that these phenotypes are in part associated with spontaneaous mutations in an Iron-Sulfur Cluster. Overall, the data provided here allowed a better understanding of the adaptive mechanisms likely developed by P. phytofirmans PsJN in heterogeneous environment.
- Subjects :
- 0301 basic medicine
[SDV]Life Sciences [q-bio]
Arabidopsis
antigenic variation
Plant Roots
Applied Microbiology and Biotechnology
biofilm
Paraburkholderia phytofirmans
Defensins
iron-sulfur cluster
necrotrophic pathogens
Environmental Microbiology
Plant defense against herbivory
static cultures
Arabidopsis thaliana
Plant Immunity
ComputingMilieux_MISCELLANEOUS
Genetics
Ecology
biology
Burkholderiaceae
Quorum Sensing
phenotypic variation
Carbon-Sulfur Lyases
defense responses
competition
Biotechnology
phase variation
030106 microbiology
arabidopsis-thaliana
03 medical and health sciences
Bacterial Proteins
growth promotion
Stress, Physiological
plant defense
HSP70 Heat-Shock Proteins
Gene
Phase variation
Whole Genome Sequencing
Arabidopsis Proteins
fungi
Wild type
Biofilm
root-colonization
biology.organism_classification
030104 developmental biology
colony morphology
Biofilms
Mutation
biology.protein
burkholderia-cepacia complex
Flagellin
Food Science
Subjects
Details
- Language :
- English
- ISSN :
- 00992240 and 10985336
- Database :
- OpenAIRE
- Journal :
- Applied and Environmental Microbiology, Applied and Environmental Microbiology, 2019, 85 (11), pp.UNSP e02670--18. ⟨10.1128/AEM.02670-18⟩, Applied and Environmental Microbiology, American Society for Microbiology, 2019, 85 (11), ⟨10.1128/AEM.02670-18⟩
- Accession number :
- edsair.doi.dedup.....eeefccd112307f4e2f7756cdd7844a3c