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A novel flow-cytometry-based assay for quantification of affinity and avidity changes of integrins
- Source :
- Journal of Immunological Methods. 310:67-77
- Publication Year :
- 2006
- Publisher :
- Elsevier BV, 2006.
-
Abstract
- Cell adhesion plays an important role in cell-cell contact formation and cell migration. Thus, the assessment of cellular adhesiveness is one important feature when studying cell-mediated immune responses. The interaction of lymphocytes with other cell types such as antigen-presenting cells or vascular-endothelial cells occurs via adhesion molecules including L-selectin, VCAM-1 or ICAM-1. There are principally two mechanisms by which cell adhesion can be enhanced: namely changes in the affinity or avidity of receptor interactions. Conventional plate-based adhesion assays detect both forms. However, they do not permit discrimination between affinity- and avidity-mediated changes in the adhesiveness. Moreover, analysis of cell subpopulations requires cell separation prior to performance of the adhesion assay. Conventional flow-cytometry-based tests make it possible to determine changes in the affinity of integrins at the single cell level. However, they fail to quantify avidity-mediated adhesiveness. Here we describe a novel flow-cytometry-based assay, which allows the detection of both integrin-mediated affinity as well as avidity changes at the single cell level. This opens up the possibility of precisely characterizing the adhesive capacity of subpopulations in heterogeneous cell populations.
- Subjects :
- Cell type
medicine.diagnostic_test
biology
Cell adhesion molecule
T-Lymphocytes
Immunology
Integrin
Cell
Antibody Affinity
Cell migration
Flow Cytometry
Intercellular Adhesion Molecule-1
Flow cytometry
Cell biology
medicine.anatomical_structure
Cell Adhesion
medicine
biology.protein
Humans
Immunology and Allergy
Avidity
Cell adhesion
Immunoglobulin Fragments
Subjects
Details
- ISSN :
- 00221759
- Volume :
- 310
- Database :
- OpenAIRE
- Journal :
- Journal of Immunological Methods
- Accession number :
- edsair.doi.dedup.....eeeed1739933a9115b1822de5aab04f5