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Prepuberal stimulation of 5-HT7-R by LP-211 in a rat model of hyper-activity and attention-deficit: permanent effects on attention, brain amino acids and synaptic markers in the fronto-striatal interface
- Source :
- PLoS ONE, PloS one 9 (2014). doi:10.1371/journal.pone.0083003, info:cnr-pdr/source/autori:Ruocco, Lucia A.; Treno, Concetta; Carnevale, Ugo A. Gironi; Arra, Claudio; Boatto, Gianpiero; Nieddu, Maria; Pagano, Cristina; Illiano, Placido; Barbato, Fabiana; Tino, Angela; Carboni, Ezio; Laviola, Giovanni; Lacivita, Enza; Leopoldo, Marcello; Adriani, Walter; Sadile, Adolfo G./titolo:Prepuberal Stimulation of 5-HT7-R by LP-211 in a Rat Model of Hyper-Activity and Attention-Deficit: Permanent Effects on Attention, Brain Amino Acids and Synaptic Markers in the Fronto-Striatal Interface/doi:10.1371%2Fjournal.pone.0083003/rivista:PloS one/anno:2014/pagina_da:/pagina_a:/intervallo_pagine:/volume:9, PLoS ONE, Vol 9, Iss 4, p e83003 (2014)
- Publication Year :
- 2013
-
Abstract
- The cross-talk at the prefronto-striatal interface involves excitatory amino acids, different receptors, transducers and modulators. We investigated long-term effects of a prepuberal, subchronic 5-HT7-R agonist (LP-211) on adult behaviour, amino acids and synaptic markers in a model for Attention-Deficit/Hyperactivity Disorder (ADHD). Naples High Excitability rats (NHE) and their Random Bred controls (NRB) were daily treated with LP-211 in the 5th and 6th postnatal week. One month after treatment, these rats were tested for indices of activity, non selective (NSA), selective spatial attention (SSA) and emotionality. The quantity of L-Glutamate (L-Glu), L-Aspartate (L-Asp) and L-Leucine (L-Leu), dopamine transporter (DAT), NMDAR1 subunit and CAMKII alpha, were assessed in prefrontal cortex (PFC), dorsal (DS) and ventral striatum (VS), for their role in synaptic transmission, neural plasticity and information processing. Prepuberal LP-211 (at lower dose) reduced horizontal activity and (at higher dose) increased SSA, only for NHE but not in NRB rats. Prepuberal LP-211 increased, in NHE rats, L-Glu in the PFC and L-Asp in the VS (at 0.250 mg/kg dose), whereas (at 0.125 mg/kg dose) it decreased L-Glu and L-Asp in the DS. The L-Glu was decreased, at 0.125 mg/kg, only in the VS of NRB rats. The DAT levels were decreased with the 0.125 mg/kg dose (in the PFC), and increased with the 0.250 mg/kg dose (in the VS), significantly for NHE rats. The basal NMDAR1 level was higher in the PFC of NHE than NRB rats; LP-211 treatment (at 0.125 mg/kg dose) decreased NMDAR1 in the VS of NRB rats. This study represents a starting point about the impact of developmental 5-HT7-R activation on neuro-physiology of attentive processes, executive functions and their neural substrates.
- Subjects :
- Male
Social Sciences
Stimulation
Biochemistry
Mechanical Treatment of Specimens
Piperazines
Rats, Sprague-Dawley
Behavioral Neuroscience
Molecular Cell Biology
Medicine and Health Sciences
Psychology
Attention
Sexual Maturation
Amino Acids
Prefrontal cortex
Multidisciplinary
biology
Systems Biology
Neurochemistry
Neurotransmitters
medicine.anatomical_structure
Electroporation
Specimen Disruption
Medicine
Neurochemicals
Research Article
Agonist
medicine.medical_specialty
CHIM/08 Chimica farmaceutica
medicine.drug_class
Science
Cognitive Neuroscience
Neurotransmission
Research and Analysis Methods
Receptors, N-Methyl-D-Aspartate
Model Organisms
Internal medicine
Mental Health and Psychiatry
medicine
Genetics
Animals
5-HT receptor
Dopamine transporter
Brain Chemistry
Dopamine Plasma Membrane Transport Proteins
Behavior
Ventral striatum
Cognitive Psychology
Biology and Life Sciences
Cell Biology
Corpus Striatum
Rats
Disease Models, Animal
Endocrinology
Attention Deficit Disorder with Hyperactivity
Specimen Preparation and Treatment
Receptors, Serotonin
biology.protein
Cognitive Science
Serotonin
Calcium-Calmodulin-Dependent Protein Kinase Type 2
Neuroscience
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 9
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.doi.dedup.....eede6241e2b5851ad754335887a45830