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Practical implications of erythromycin resistance gene diversity on surveillance and monitoring of resistance
- Source :
- FEMS Microbiology Ecology
- Publication Year :
- 2018
- Publisher :
- Oxford University Press (OUP), 2018.
-
Abstract
- Use of antibiotics in human and animal medicine has applied selective pressure for the global dissemination of antibiotic-resistant bacteria. Therefore, it is of interest to develop strategies to mitigate the continued amplification and transmission of resistance genes in environmental reservoirs such as farms, hospitals and watersheds. However, the efficacy of mitigation strategies is difficult to evaluate because it is unclear which resistance genes are important to monitor, and which primers to use to detect those genes. Here, we evaluated the diversity of one type of macrolide antibiotic resistance gene (erm) in one type of environment (manure) to determine which primers would be most informative to use in a mitigation study of that environment. We analyzed all known erm genes and assessed the ability of previously published erm primers to detect the diversity. The results showed that all known erm resistance genes group into 66 clusters, and 25 of these clusters (40%) can be targeted with primers found in the literature. These primers can target 74%–85% of the erm gene diversity in the manures analyzed.<br />We evaluate and identify the most effective primer gene targets to detect erythromycin resistance originating from manure sources.
- Subjects :
- 0301 basic medicine
Farms
medicine.drug_class
030106 microbiology
Antibiotics
Erythromycin
Biology
Applied Microbiology and Biotechnology
Microbiology
target
resistance
03 medical and health sciences
Bacterial Proteins
antibiotic
Drug Resistance, Bacterial
Genetic variation
medicine
Animals
genes
Practical implications
Gene
DNA Primers
Genetics
Bacteria
Ecology
Resistance (ecology)
Tetracycline Resistance
Genetic Variation
Methyltransferases
biochemical phenomena, metabolism, and nutrition
Anti-Bacterial Agents
Erythromycin resistance
Manure
surveillance
Surveillance and monitoring
environment
Research Article
medicine.drug
Subjects
Details
- ISSN :
- 15746941
- Volume :
- 94
- Database :
- OpenAIRE
- Journal :
- FEMS Microbiology Ecology
- Accession number :
- edsair.doi.dedup.....eedce0bb89a0d5badf23e6c746afbeaf