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Cannabisin B induces autophagic cell death by inhibiting the AKT/mTOR pathway and S phase cell cycle arrest in HepG2 cells
- Source :
- Food chemistry. 138(2-3)
- Publication Year :
- 2012
-
Abstract
- This study investigates the anticancer properties of cannabisin B, purified from hempseed hull, in HepG2 human hepatoblastoma cells. The results indicate that cannabisin B significantly inhibited cell proliferation by inducing autophagic cell death rather than typical apoptosis. Cell viability transiently increased upon the addition of a low concentration of cannabisin B but decreased upon the addition of high concentrations. Cannabisin B-induced changes in cell viability were completely inhibited by pre-treatment with 3-methyladenine (3-MA), indicating that the induction of autophagy by cannabisin B caused cell death. Additionally, cannabisin B induced S phase cell cycle arrest in a dose-dependent manner. Moreover, cannabisin B was found to inhibit survival signaling by blocking the activation of AKT and down-stream targets of the mammalian target of rapamycin (mTOR). These findings suggest that cannabisin B possesses considerable antiproliferative activity and that it may be utilised as a promising chemopreventive agent against hepatoblastoma disease.
- Subjects :
- Programmed cell death
Cell cycle checkpoint
Cell growth
Plant Extracts
TOR Serine-Threonine Kinases
Autophagy
Down-Regulation
General Medicine
Hep G2 Cells
Biology
Analytical Chemistry
Cell biology
Apoptosis
S Phase Cell Cycle Checkpoints
Seeds
Humans
Viability assay
Protein kinase B
Proto-Oncogene Proteins c-akt
PI3K/AKT/mTOR pathway
Food Science
Cannabis
Signal Transduction
Subjects
Details
- ISSN :
- 18737072
- Volume :
- 138
- Issue :
- 2-3
- Database :
- OpenAIRE
- Journal :
- Food chemistry
- Accession number :
- edsair.doi.dedup.....eed3f9c938eb9b05e7d94a654082e01f