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Molecular Umbrella Conjugate for the Ocular Delivery of siRNA
- Source :
- Bioconjugate Chemistry
- Publication Year :
- 2014
- Publisher :
- American Chemical Society (ACS), 2014.
-
Abstract
- The synthesis, computer modeling, and biological activity of an octawalled molecular umbrella short interfacing RNA (siRNA) conjugate is described. This molecular umbrella-siRNA conjugate exhibited mRNA knockdown activity in vitro in the absence of a transfection reagent. Evaluation of this molecular umbrella conjugate in vivo, using the rat eye via intravitreal injection, resulted in sequence specific mRNA knockdown in the retina with no obvious signs of toxicity, as judged by ophthalmic examination.
- Subjects :
- congenital, hereditary, and neonatal diseases and abnormalities
Biomedical Engineering
Pharmaceutical Science
Bioengineering
Molecular Dynamics Simulation
Pharmacology
Eye
In vivo
Humans
RNA, Small Interfering
Drug Carriers
Messenger RNA
Gene knockdown
Chemistry
Communication
Drug Administration Routes
Organic Chemistry
technology, industry, and agriculture
RNA
Biological activity
Transfection
Molecular biology
In vitro
HEK293 Cells
lipids (amino acids, peptides, and proteins)
Biotechnology
Conjugate
Subjects
Details
- ISSN :
- 15204812 and 10431802
- Volume :
- 25
- Database :
- OpenAIRE
- Journal :
- Bioconjugate Chemistry
- Accession number :
- edsair.doi.dedup.....eed0788df98a80639c2be2827b3f43b0
- Full Text :
- https://doi.org/10.1021/bc400506m