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Molecular mechanisms involved in increased expression of a cytochrome P450 responsible for pyrethroid resistance in the housefly, Musca domestica
Molecular mechanisms involved in increased expression of a cytochrome P450 responsible for pyrethroid resistance in the housefly, Musca domestica
- Source :
- Insect Molecular Biology. 4:135-140
- Publication Year :
- 1995
- Publisher :
- Wiley, 1995.
-
Abstract
- Cytochrome P450 Ipr is a developmentally regulated P450 responsible for monooxygenase-mediated pyrethroid resistance in the LPR strain of housefly. CYP6D1, the gene coding for P450 Ipr, has recently been sequenced. We investigated the molecular basis for CYP6D1-mediated pyrethroid resistance by comparison of mRNA levels, gene sequences, and gene copy number between LPR and pyrethroid susceptible strains of housefly. CYP6D1 mRNA levels were elevated in the LPR strain to a similar level as P450 Ipr protein, suggesting that over-expression of this important P450 in the resistant strain is probably due to increased transcription. Southern blots of susceptible and LPR strain DNA suggest that gene amplification is probably not involved in the increased expression of CYP6D1 protein. Five alleles of CYP6D1 were discovered and their possible role in resistance is discussed.
- Subjects :
- Molecular Sequence Data
Gene Expression Regulation, Enzymologic
Insecticide Resistance
chemistry.chemical_compound
Cytochrome P-450 Enzyme System
Transcription (biology)
Houseflies
Pyrethrins
Gene duplication
Genetics
Animals
Cytochrome P450 Family 6
Amino Acid Sequence
RNA, Messenger
Housefly
Molecular Biology
Gene
Southern blot
Regulation of gene expression
Polymorphism, Genetic
Pyrethroid
Base Sequence
biology
Cytochrome P450
biology.organism_classification
Molecular biology
chemistry
Insect Science
biology.protein
Insect Proteins
Subjects
Details
- ISSN :
- 13652583 and 09621075
- Volume :
- 4
- Database :
- OpenAIRE
- Journal :
- Insect Molecular Biology
- Accession number :
- edsair.doi.dedup.....eeb251f5fea41d1053707ceeb6d1c262
- Full Text :
- https://doi.org/10.1111/j.1365-2583.1995.tb00018.x