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Intracellular stimulation of mast cells with guanine nucleotides mimic antigenic stimulation
- Source :
- FEBS letters. 216(1)
- Publication Year :
- 1987
-
Abstract
- Exocytosis was followed in single rat peritoneal mast cells, by measuring the cell membrane capacitance using circuit analysis and patch-clamp techniques. After antigenic stimulation or intracellular perfusion with guanine nucleotides, exocytosis followed a time course characterized by a lag period d, area expansion factor A, and a time constant τ. We suggest that A depends entirely on the cell's morphology, d reflects the properties of a GTP-binding regulatory protein that appears to rate limit the response and τ is due to an independent and yet unknown process. In contrast, cells stimulated by compound 48/80 can respond without a measurable delay and degranulate within 2 s, suggesting that this compound acts at a site after the GTP-binding regulatory protein.
- Subjects :
- Guanine
Biophysics
Stimulation
Biology
Biochemistry
Exocytosis
Mast cell
Membrane Potentials
Cell membrane
chemistry.chemical_compound
Guanine nucleotide
Structural Biology
GTP-Binding Proteins
Genetics
medicine
Animals
p-Methoxy-N-methylphenethylamine
Patch clamp
Mast Cells
Molecular Biology
48/80
HEPES
Cell Biology
Molecular biology
Guanine Nucleotides
Rats
medicine.anatomical_structure
chemistry
Antigen
Patch-clamp
Intracellular
Subjects
Details
- ISSN :
- 00145793
- Volume :
- 216
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- FEBS letters
- Accession number :
- edsair.doi.dedup.....ee92f74335d8490f0dd93dead0cf6d6b