Back to Search
Start Over
RelA-Mediated BECN1 Expression Is Required for Reactive Oxygen Species-Induced Autophagy in Oral Cancer Cells Exposed to Low-Power Laser Irradiation
- Source :
- PLoS ONE, Vol 11, Iss 9, p e0160586 (2016), PLoS ONE
- Publication Year :
- 2016
- Publisher :
- Public Library of Science (PLoS), 2016.
-
Abstract
- Low-power laser irradiation (LPLI) is a non-invasive and safe method for cancer treatment that alters a variety of physiological processes in the cells. Autophagy can play either a cytoprotective role or a detrimental role in cancer cells exposed to stress. The detailed mechanisms of autophagy and its role on cytotoxicity in oral cancer cells exposed to LPLI remain unclear. In this study, we showed that LPLI at 810 nm with energy density 60 J/cm2 increased the number of microtubule associated protein 1 light chain 3 (MAP1LC3) puncta and increased autophagic flux in oral cancer cells. Moreover, reactive oxygen species (ROS) production was induced, which increased RelA transcriptional activity and beclin 1 (BECN1) expression in oral cancer cells irradiated with LPLI. Furthermore, ROS scavenger or knockdown of RelA diminished LPLI-induced BECN1 expression and MAP1LC3-II conversion. In addition, pharmacological and genetic ablation of autophagy significantly enhanced the effects of LPLI-induced apoptosis in oral cancer cells. These results suggest that autophagy may be a resistant mechanism for LPLI-induced apoptosis in oral cancer cells.
- Subjects :
- 0301 basic medicine
Cancer Treatment
lcsh:Medicine
Apoptosis
Biochemistry
Oxidative Damage
0302 clinical medicine
Medicine and Health Sciences
Post-Translational Modification
Phosphorylation
lcsh:Science
Cytotoxicity
Staining
chemistry.chemical_classification
Gene knockdown
Multidisciplinary
Cell Death
NF-kappa B
Cell Staining
BECN1
Cell biology
Oncology
Optical Equipment
Cell Processes
030220 oncology & carcinogenesis
Engineering and Technology
Beclin-1
Mouth Neoplasms
Research Article
Autophagic Cell Death
Immunoblotting
Molecular Probe Techniques
Equipment
Biology
Research and Analysis Methods
03 medical and health sciences
Autophagy
Humans
Low-Level Light Therapy
Molecular Biology Techniques
Molecular Biology
Transcription factor
Reactive oxygen species
Lasers
lcsh:R
Transcription Factor RelA
Biology and Life Sciences
Proteins
Cell Biology
030104 developmental biology
chemistry
Specimen Preparation and Treatment
Cancer cell
lcsh:Q
Reactive Oxygen Species
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- PLOS ONE
- Accession number :
- edsair.doi.dedup.....ee87b54c25e61206c8a28b8525313d68