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Isothermal amplification of specific DNA molecules inside giant unilamellar vesicles

Authors :
Shin Ichiro M. Nomura
Ken Komiya
Yusuke Sato
Ibuki Kawamata
Satoshi Murata
Source :
Chemical Communications. 55:9084-9087
Publication Year :
2019
Publisher :
Royal Society of Chemistry (RSC), 2019.

Abstract

An isothermal amplification circuit for specific DNA molecules was implemented in giant unilamellar vesicles. Using this circuit, over 5000-fold amplification of output DNAs was achieved, and the amplification behaviour depended on the concentration of input signal DNAs in a cell-sized compartment. Moreover, initiation of the amplification by photo-stimulation was demonstrated.

Details

ISSN :
1364548X and 13597345
Volume :
55
Database :
OpenAIRE
Journal :
Chemical Communications
Accession number :
edsair.doi.dedup.....ee816f6a871c183feed07a3d462e7908