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Isothermal amplification of specific DNA molecules inside giant unilamellar vesicles
- Source :
- Chemical Communications. 55:9084-9087
- Publication Year :
- 2019
- Publisher :
- Royal Society of Chemistry (RSC), 2019.
-
Abstract
- An isothermal amplification circuit for specific DNA molecules was implemented in giant unilamellar vesicles. Using this circuit, over 5000-fold amplification of output DNAs was achieved, and the amplification behaviour depended on the concentration of input signal DNAs in a cell-sized compartment. Moreover, initiation of the amplification by photo-stimulation was demonstrated.
- Subjects :
- Surface Properties
Loop-mediated isothermal amplification
010402 general chemistry
01 natural sciences
Signal
Catalysis
chemistry.chemical_compound
Materials Chemistry
Molecule
Particle Size
Unilamellar Liposomes
010405 organic chemistry
Vesicle
technology, industry, and agriculture
Metals and Alloys
DNA
General Chemistry
Compartment (chemistry)
0104 chemical sciences
Surfaces, Coatings and Films
Electronic, Optical and Magnetic Materials
chemistry
Ceramics and Composites
Biophysics
Nucleic Acid Amplification Techniques
Subjects
Details
- ISSN :
- 1364548X and 13597345
- Volume :
- 55
- Database :
- OpenAIRE
- Journal :
- Chemical Communications
- Accession number :
- edsair.doi.dedup.....ee816f6a871c183feed07a3d462e7908