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Sugarcane genome architecture decrypted with chromosome-specific oligo probes
- Source :
- Plant Journal, Plant Journal, Wiley, In press, pp.1-13. ⟨10.1111/tpj.14881⟩
- Publication Year :
- 2020
-
Abstract
- Sugarcane (Saccharum spp.) is probably the crop with the most complex genome. Modern cultivars (2n = 100–120) are highly polyploids and aneuploids derived from interspecific hybridization between Saccharum officinarum (2n = 80) and Saccharum spontaneum (2n = 40–128). Chromosome‐specific oligonucleotide probes were used in combination with genomic in situ hybridization to analyze the genome architecture of modern cultivars and representatives of their parental species. The results validated a basic chromosome number of x = 10 for S. officinarum . In S. spontaneum , rearrangements occurred from a basic chromosome of x = 10, probably in the Northern part of India, in two steps leading to x = 9 and then x = 8. Each step involved three chromosomes that were rearranged into two. Further polyploidization led to the wide geographical extension of clones with x = 8. We showed that the S. spontaneum contribution to modern cultivars originated from cytotypes with x = 8 and varied in proportion between cultivars (13–20%). Modern cultivars had mainly 12 copies for each of the first four basic chromosomes, and a more variable number for those basic chromosomes whose structure differs between the two parental species. One−four of these copies corresponded to entire S. spontaneum chromosomes or interspecific recombinant chromosomes. In addition, a few inter‐chromosome translocations were revealed. The new information and cytogenetic tools described in this study substantially improve our understanding of the extreme level of complexity of modern sugarcane cultivar genomes.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Phylogénie
[SDV]Life Sciences [q-bio]
chromosome mapping [EN]
Hybride
Chromosomal translocation
Plant Science
01 natural sciences
Genome
F30 - Génétique et amélioration des plantes
Saccharum officinarum
Cultivar
In Situ Hybridization, Fluorescence
Genetics
Saccharum spontaneum
Chromosome Mapping
food and beverages
Polyploïdie
Saccharum
GISH
chromosome-specific oligo FISH
Genome, Plant
interspecific hybrid
Biology
Chromosomes, Plant
Polyploidy
03 medical and health sciences
Polyploid
Nombre chromosomique
Cytogénétique
Génome
polyploid
Chromosome
Cell Biology
Aneuploidy
biology.organism_classification
chromosome rearrangements
030104 developmental biology
Oligonucleotide Probes
010606 plant biology & botany
Subjects
Details
- Language :
- English
- ISSN :
- 09607412 and 1365313X
- Database :
- OpenAIRE
- Journal :
- Plant Journal, Plant Journal, Wiley, In press, pp.1-13. ⟨10.1111/tpj.14881⟩
- Accession number :
- edsair.doi.dedup.....ee7934cb61b305a2149570568f67412e