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Immunomodulating effects of cefodizime on Klebsiella pneumoniae-stimulated neutrophils
- Source :
- Immunobiology. 209:277-282
- Publication Year :
- 2004
- Publisher :
- Elsevier BV, 2004.
-
Abstract
- To explore the immunoregulating effects of cefodizime on neutrophils and its mechanisms, we detected the expression of some cytokines secreted by neutrophils after heat-killed Klebsiella pneumoniae induced inflammation. We analyzed the changes of signal transduction in this process by detecting the mRNA expression of toll like receptor 4 (TLR4) and the inhibitor factor of kappaBalpha (I-kappaBalpha) expressed by neutrophils. The activity of nuclear factor kappaB (NF-kappaB) in neutrophils was also assayed by electrophoretic mobility shift assay (EMSA). We found cefodizime increased neutrophil production of TNF-alpha, IL-1beta in the early stage of inflammation, which was in agreement with the enhanced mRNA expression of TLR4 and DNA-binding activity of NF-kappaB. Taken together, the immunomodulating effects of cefodizime on cytokine production of K. pneumoniae stimulated neutrophils is possibly due to its regulation of TLR4 mRNA expression and DNA binding activities of NF-kappaB through the LPS-TLR4-NF-kappaB pathway.
- Subjects :
- Lipopolysaccharides
Neutrophils
medicine.medical_treatment
Immunology
Receptors, Cell Surface
Inflammation
Cefotaxime
Biology
Neutrophil Activation
Microbiology
Cefodizime
Mice
chemistry.chemical_compound
medicine
Animals
Immunology and Allergy
Electrophoretic mobility shift assay
Membrane Glycoproteins
Toll-Like Receptors
NF-kappa B
NF-κB
Hematology
Molecular biology
Anti-Bacterial Agents
Toll-Like Receptor 4
Klebsiella pneumoniae
Cytokine
chemistry
TLR4
Cytokines
Tumor necrosis factor alpha
medicine.symptom
Signal transduction
Signal Transduction
medicine.drug
Subjects
Details
- ISSN :
- 01712985
- Volume :
- 209
- Database :
- OpenAIRE
- Journal :
- Immunobiology
- Accession number :
- edsair.doi.dedup.....ee6ff41b71bb3c9b668e069809ab2171