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Modulation of NF-κB activation by resveratrol in LPS treated human intestinal cells results in downregulation of PGE2 production and COX-2 expression
- Source :
- Toxicology in vitro : an international journal published in association with BIBRA. 26(7)
- Publication Year :
- 2012
-
Abstract
- Resveratrol is a natural phytoalexin present in a variety of plant species, such as grapes and red wine, that is well known for its anti-inflammatory effects. In addition, a cancer chemotherapeutic activity of resveratrol has been described. Here we evaluated the effect of resveratrol on COX-2 and prostaglandin E(2) production in human intestinal cells Caco-2 cells treated with lipopolysaccharide (LPS). Resveratrol concentration-dependently inhibited the expression of COX-2 mRNA in the LPS-treated cells, as well as protein expression, resulting in a decreased production of PGE(2). In order to investigate the mechanisms through which resveratrol exhibited these anti-inflammatory effects, we examined the activation of IκB in LPS-stimulated intestinal cells. Results demonstrated that resveratrol inhibited the translocation of NF-κB p65 subunits from the cytosol to the nucleus, which correlated with its inhibitory effects on IκBα phosphorylation and degradation. These results suggest that the down-regulation of COX-2 and PGE(2) by resveratrol may be related to NF-κB inhibition through the negative regulation of IKK phosphorylation in intestinal cells.
- Subjects :
- Lipopolysaccharides
Cell Survival
medicine.medical_treatment
Down-Regulation
IκB kinase
Pharmacology
Resveratrol
Toxicology
Antioxidants
Dinoprostone
Gene Expression Regulation, Enzymologic
Cell Line
chemistry.chemical_compound
Downregulation and upregulation
Stilbenes
medicine
Humans
skin and connective tissue diseases
Cells, Cultured
chemistry.chemical_classification
Chemistry
Phytoalexin
NF-kappa B
food and beverages
NF-κB
General Medicine
IκBα
Enterocytes
Biochemistry
Cell culture
Cyclooxygenase 2
Caco-2 Cells
Prostaglandin E
Subjects
Details
- ISSN :
- 18793177
- Volume :
- 26
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Toxicology in vitro : an international journal published in association with BIBRA
- Accession number :
- edsair.doi.dedup.....ee651f7598bb73e2b11c18f2a879c76c