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N‐methyl‐D‐aspartic acid/glycine interactions on the control of 5‐hydroxytryptamine release in raphe primary cultures
- Source :
- Journal of Neurochemistry, Journal of Neurochemistry, Wiley, 1993, 61 (5), pp.1692--1697, HAL, Journal of Neurochemistry, 1993, 61 (5), pp.1692--1697
- Publication Year :
- 1993
- Publisher :
- HAL CCSD, 1993.
-
Abstract
- International audience; Glutamic acid and glycine were quantified in cells and medium of cultured rostral rhombencephalic neurons derived from fetal rats. In the presence of 1 mM Mg2+, NMDA (50 microM) significantly stimulated (by 69%) release of newly synthesized 5‐[3H]hydroxytryptamine ([3H]5‐HT). D‐2‐Amino‐5‐phosphonopentanoate (AP‐5; 50 microM) blocked the stimulatory effect of NMDA. AP‐5 by itself inhibited [3H]5‐HT release (by 25%), suggesting a tonic control of 5‐HT by glutamate. In the absence of Mg2+, basal [3H]5‐HT release was 60% higher as compared with release with Mg2+. AP‐5 blocked the increased [3H]5‐HT release observed without Mg2+, suggesting that this effect was due to the stimulation of NMDA receptors by endogenous glutamate. Glycine (100 microM) inhibited [3H]5‐HT release in the absence of Mg2+. Strychnine (50 microM) blocked the inhibitory effect of glycine, indicating an action through strychnine‐sensitive inhibitory glycine receptors. The [3H]5‐HT release stimulated by NMDA was unaffected by glycine. In contrast, when tested in the presence of strychnine, glycine increased NMDA‐evoked [3H]5‐HT release (by 22%), and this effect was prevented by a selective antagonist of the NMDA‐associated glycine receptor, 7‐chlorokynurenate (100 microM). 7‐Chlorokynurenate by itself induced a drastic decrease in [3H]5‐HT release, indicating that under basal conditions these sites were stimulated by endogenous glycine. These results indicate that NMDA stimulated [3H]5‐HT release in both the presence or absence of Mg2+. Use of selective antagonists allowed differentiation of a strychnine‐sensitive glycine response (inhibition of [3H]5‐HT release) from a 7‐chlorokynurenate‐sensitive response (potentiation of NMDA‐evoked [3H]5‐HT release).
- Subjects :
- medicine.medical_specialty
Serotonin
N-Methylaspartate
[SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
Glycine
N-Methyl-D-aspartic acid
Glutamic Acid
Gestational Age
Biology
Inhibitory postsynaptic potential
Kynurenic Acid
Biochemistry
Receptors, N-Methyl-D-Aspartate
Rats, Sprague-Dawley
Cellular and Molecular Neuroscience
chemistry.chemical_compound
Receptors, Glycine
Glutamates
Pregnancy
Internal medicine
medicine
Animals
Neurotransmitter
Glycine receptor
Cells, Cultured
Glutamate receptor
[SDV.NEU.NB] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology
Valine
Strychnine
Glutamic acid
Embryo, Mammalian
Rats
Rhombencephalon
Kinetics
Endocrinology
chemistry
NMDA receptor
Raphe Nuclei
Female
Subjects
Details
- Language :
- English
- ISSN :
- 00223042 and 14714159
- Database :
- OpenAIRE
- Journal :
- Journal of Neurochemistry, Journal of Neurochemistry, Wiley, 1993, 61 (5), pp.1692--1697, HAL, Journal of Neurochemistry, 1993, 61 (5), pp.1692--1697
- Accession number :
- edsair.doi.dedup.....ee402bbf79347f75473d42153a81ddfd