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Metagenomic analysis of the effects of toll-like receptors on bacterial infection in the peritoneal cavity following cecum ligation and puncture in mice
- Source :
- PLoS ONE, PLoS ONE, Vol 14, Iss 7, p e0220398 (2019)
- Publication Year :
- 2019
- Publisher :
- Public Library of Science, 2019.
-
Abstract
- ObjectiveTo establish the composition of bacteria in mice following cecum ligation and puncture (CLP) through metagenomic analysis and investigate the role of TLRs on the composition of bacteria.MethodsTotal DNA extraction was done from the ascites, blood, and fecal samples from C57BL/6 mice sacrificed at 0, 4, 8, and 16 h, as well as from Tlr2-/-, Tlr4-/-, Tlr5-/-, and NF-κB-/-mice sacrificed at 16 h following CLP. Amplification of the V3-V4 regions of the bacterial 16S rRNA genes by PCR and the Illumina MiSeq sequencer was used for deep sequencing. Hierarchical clustering of the isolates was performed with Ward's method using Euclidean distances. The relative abundance according to operational taxonomic unit (OTU) number or taxa was used to compare the richness among subgroups in the experiments.ResultsThere were 18 taxa that had significantly different abundances among the different samples of the C57BL/6 mice at 16 h following CLP. Various dynamic changes in the infectious bacteria inside the peritoneal cavity after CLP were found. While knockout of Tlr5 and NF-κB impaired the ability of bacterial clearance inside the peritoneal cavity for some kinds of bacteria found in the C57BL/6 mice, the knockout of Tlr4 enhanced clearance for other kinds of bacteria, and they presented excessive abundance in the peritoneal cavity despite their scarce abundance in the stool.ConclusionNF-κB and TLRs are involved in bacterial clearance and in the expression pattern of the bacterial abundance inside the peritoneal cavity during polymicrobial infection.
- Subjects :
- 0301 basic medicine
Operational taxonomic unit
Physiology
Pathology and Laboratory Medicine
Immune Receptors
Biochemistry
Cecum
Feces
Mice
RNA, Ribosomal, 16S
Ascites
Medicine and Health Sciences
Toll-like Receptors
Peritoneal Cavity
Phylogeny
Multidisciplinary
Immune System Proteins
NF-kappa B
High-Throughput Nucleotide Sequencing
Genomics
Body Fluids
Nucleic acids
medicine.anatomical_structure
Blood
Ribosomal RNA
Medical Microbiology
Gene Knockdown Techniques
Medicine
medicine.symptom
Anatomy
Research Article
Signal Transduction
DNA, Bacterial
Cellular structures and organelles
Science
030106 microbiology
Immunology
Gastroenterology and Hepatology
Microbial Genomics
Biology
Microbiology
DNA, Ribosomal
Sepsis
03 medical and health sciences
Peritoneal cavity
Signs and Symptoms
Diagnostic Medicine
medicine
Genetics
Animals
Non-coding RNA
Bacteria
Organisms
Biology and Life Sciences
Proteins
Cell Biology
medicine.disease
biology.organism_classification
DNA extraction
Mice, Inbred C57BL
030104 developmental biology
RNA
Metagenomics
Microbiome
Ribosomes
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 14
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....ee21cc46337672b2dfc46449ab53f8cf