Back to Search
Start Over
Probiotics and gut health in infants: A preliminary case–control observational study about early treatment with Lactobacillus reuteri DSM 17938
- Source :
- Clinica Chimica Acta. 451:82-87
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- Aims We performed this case–control observational study to evaluate the effects of early administration of Lactobacillus reuteri DSM 17938 on microbial composition in infants' gastrointestinal tract. Methods Early fecal microbiota composition was analyzed by using selective and differential cultural methods. Genomic DNA from positive Escherichia coli and Cronobacter sakazakii colonies was extracted and DNA was processed by multiplex PCR assay. Results Fecal samples of 30 hospitalized infants who previously received probiotics and 30 not receiving probiotics were analyzed. We find that the two groups showed differences in gut microbial strains composition and richness. Infant treated with probiotics have a lower total anaerobic gram negative counts ( p = 0.03) and a higher total anaerobic gram-positive counts ( p = 0.02). Enterobacteriaceae and enterococci were significantly higher ( p = 0.04) in the control group. No significant differences were observed for total aerobic counts, lactobacilli and bifidobacteria. C. sakazaki was found only in one infant recruited in the control group. Infants not previously treated with probiotics showed a higher colonization by diarrheagenic E. coli (EPEC) ( p = 0.04). Conclusions Our findings enhanced our understanding of the effects of probiotics on gut health in pediatric subjects. Early administration of L. reuteri in infancy could improve gut health by reducing pathogens colonization.
- Subjects :
- DNA, Bacterial
Limosilactobacillus reuteri
Male
Clinical Biochemistry
Gut flora
medicine.disease_cause
Biochemistry
law.invention
Microbiology
formula fed
Probiotic
Cronobacter sakazakii
law
Escherichia coli
medicine
Humans
Feces
Gastrointestinal tract
gut microbiota
biology
Probiotics
Biochemistry (medical)
Infants, Lactobacillus reuteri DSM 17938, formula fed, gut microbiota, microbial pathogens, probiotic
Infant
General Medicine
biology.organism_classification
Enterobacteriaceae
Gastrointestinal Microbiome
Lactobacillus reuteri
Gastrointestinal Tract
Case-Control Studies
Lactobacillus reuteri DSM 17938
Female
microbial pathogens
Infants
probiotic
Subjects
Details
- ISSN :
- 00098981
- Volume :
- 451
- Database :
- OpenAIRE
- Journal :
- Clinica Chimica Acta
- Accession number :
- edsair.doi.dedup.....edf84dbd2a1037247621d9bbaf7bc4df