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A phosphatidic acid-binding lncRNA SNHG9 facilitates LATS1 liquid–liquid phase separation to promote oncogenic YAP signaling
- Source :
- Cell Res, Cell research, vol 31, iss 10
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- Long noncoding RNAs (lncRNAs) are emerging as a new class of important regulators of signal transduction in tissue homeostasis and cancer development. Liquid-liquid phase separation (LLPS) occurs in a wide range of biological processes, while its role in signal transduction remains largely undeciphered. In this study, we uncovered a lipid-associated lncRNA, small nucleolar RNA host gene 9 (SNHG9) as a tumor-promoting lncRNA driving liquid droplet formation of Large Tumor Suppressor Kinase 1 (LATS1) and inhibiting the Hippo pathway. Mechanistically, SNHG9 and its associated phosphatidic acids (PA) interact with the C-terminal domain of LATS1, promoting LATS1 phase separation and inhibiting LATS1-mediated YAP phosphorylation. Loss of SNHG9 suppresses xenograft breast tumor growth. Clinically, expression of SNHG9 positively correlates with YAP activity and breast cancer progression. Taken together, our results uncover a novel regulatory role of a tumor-promoting lncRNA (i.e., SNHG9) in signal transduction and cancer development by facilitating the LLPS of a signaling kinase (i.e., LATS1).
- Subjects :
- Cell signaling
Clinical Sciences
Phosphatidic Acids
Protein Serine-Threonine Kinases
Biology
Article
Cell Line
Cell Line, Tumor
Breast Cancer
Genetics
2.1 Biological and endogenous factors
Humans
Hippo Signaling Pathway
Aetiology
Small nucleolar RNA
Molecular Biology
Tissue homeostasis
Cancer
Biological Phenomena
Cell Proliferation
Hippo signaling pathway
Tumor
Kinase
Phosphatidic acid binding
YAP-Signaling Proteins
Cell Biology
Phosphoproteins
Cell biology
RNA
Phosphorylation
Long Noncoding
RNA, Long Noncoding
Biochemistry and Cell Biology
Signal transduction
Biotechnology
Developmental Biology
Signal Transduction
Subjects
Details
- ISSN :
- 17487838 and 10010602
- Volume :
- 31
- Database :
- OpenAIRE
- Journal :
- Cell Research
- Accession number :
- edsair.doi.dedup.....edf1b15cb834d9c1ee8466fa7f864f47